National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China.
Genes (Basel). 2022 Aug 25;13(9):1524. doi: 10.3390/genes13091524.
Saprolegniasis, which is caused by , leads to considerable economic losses. Recently, we showed that metalaxyl, bronopol and copper sulfate are good antimicrobial agents for aquaculture. In the current study, the efficacies of metalaxyl, bronopol and copper sulfate are evaluated by in vitro antimicrobial experiments, and the mechanism of action of these three antimicrobials on is explored using transcriptome technology. Finally, the potential target genes of antimicrobials on are identified by protein-protein interaction network analysis. Copper sulfate had the best inhibitory effect on , followed by bronopol. A total of 1771, 723 and 2118 DEGs upregulated and 1416, 319 and 2161 DEGs downregulated after three drug treatments (metalaxyl, bronopol and copper sulfate), separately. Additionally, KEGG pathway analysis also determined that there were 17, 19 and 13 significantly enriched metabolic pathways. PPI network analysis screened out three important proteins, and their corresponding genes were SPRG_08456, SPRG_03679 and SPRG_10775. Our results indicate that three antimicrobials inhibit growth by affecting multiple biological functions, including protein synthesis, oxidative stress, lipid metabolism and energy metabolism. Additionally, the screened key genes can be used as potential target genes of chemical antimicrobial drugs for .
水霉病由 引起，导致了相当大的经济损失。最近，我们发现金属类药剂（如甲霜灵）、季铵盐类药剂（如溴硝醇）和铜盐类药剂（如硫酸铜）对水产养殖具有良好的抗菌作用。在本研究中，我们通过体外抗菌实验评估了金属类药剂、季铵盐类药剂和铜盐类药剂的疗效，并利用转录组技术探索了这三种抗菌剂对 的作用机制。最后，通过蛋白质-蛋白质相互作用网络分析鉴定了抗菌剂对 的潜在靶标基因。硫酸铜对 的抑制效果最好，其次是溴硝醇。三种药物（金属类药剂、季铵盐类药剂和铜盐类药剂）处理后，分别有 1771、723 和 2118 个上调基因和 1416、319 和 2161 个下调基因。此外，KEGG 通路分析还确定了 17、19 和 13 个显著富集的代谢途径。PPI 网络分析筛选出三个重要蛋白，其对应的基因分别为 SPRG_08456、SPRG_03679 和 SPRG_10775。我们的研究结果表明，三种抗菌剂通过影响包括蛋白质合成、氧化应激、脂质代谢和能量代谢在内的多种生物学功能来抑制 的生长。此外，筛选出的关键基因可以作为水霉潜在的化学抗菌药物靶标基因。
