Lv Dongjun, Zhang Mingjie, Cui Jin, Li Weixue, Zhu Guohua
Department of Chemistry, School of Science, Tianjin University, Tianjin 300354, China.
Shandong Yu Hong New Pigment Co., Ltd., Dezhou 253000, China.
Materials (Basel). 2017 Feb 23;10(3):219. doi: 10.3390/ma10030219.
A new preparation method of polymer dyes was developed to improve both the grafting degree of the azo dyes onto O-carboxymethyl chitosan (OMCS) and the water solubility of prepared polymer dyes. Firstly, the coupling compound of two azo edible colorants, sunset yellow (SY) and allura red (AR), was grafted onto OMCS, and then coupled with their diazonium salt. The chemical structure of prepared polymer dyes was determined by Fourier transform-infrared spectroscopy and ¹H-NMR, and the results showed that the two azo dyes were successfully grafted onto OMCS. The grafting degree onto OMCS and the water solubility of polymer dyes were tested, and the results showed that they were both improved as expected. The UV-vis spectra analysis results showed that the prepared polymer dyes showed similar color performance with the original azo dyes. Eventually, the cytotoxicity of prepared polymer dyes was tested and compared with the original azo dyes by a cytotoxicity test on human liver cell lines LO2, and the results showed that their grafting onto OMCS significantly reduced the cytotoxicity.
为了提高偶氮染料在O-羧甲基壳聚糖（OMCS）上的接枝度以及制备的聚合物染料的水溶性，开发了一种聚合物染料的新制备方法。首先，将两种偶氮食用色素日落黄（SY）和诱惑红（AR）的偶合化合物接枝到OMCS上，然后与它们的重氮盐偶合。通过傅里叶变换红外光谱和¹H-NMR确定了制备的聚合物染料的化学结构，结果表明两种偶氮染料成功接枝到了OMCS上。测试了聚合物染料在OMCS上的接枝度和水溶性，结果表明它们均如预期得到了提高。紫外可见光谱分析结果表明，制备的聚合物染料与原始偶氮染料表现出相似的颜色性能。最后，通过对人肝癌细胞系LO2进行细胞毒性测试，测试了制备的聚合物染料的细胞毒性并与原始偶氮染料进行了比较，结果表明它们接枝到OMCS上显著降低了细胞毒性。
