Rabadan-Ros Ruben, Aznar-Cervantes Salvador, Mazón Patricia, Ros-Tarraga Patricia, De Aza Piedad N, Meseguer-Olmo Luis
Grupo de Regeneración y Reparación de Tejidos: Ortobiología, Biomateriales e Ingeniería de Tejidos, Universidad Católica San Antonio de Murcia (UCAM), Guadalupe, 30107 Murcia, Spain.
Department of Biotechnology, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), La Alberca, 30150 Murcia, Spain.
Materials (Basel). 2017 Mar 27;10(4):347. doi: 10.3390/ma10040347.
The purpose of this study was to evaluate the bioactivity and cell response of a well-characterized Nurse's A-phase (7CaO·P₂O₅·2SiO₂) ceramic and its effect compared to a control (tissue culture polystyrene-TCPS) on the adhesion, viability, proliferation, and osteogenic differentiation of MSCs in vitro. Cell proliferation (Alamar Blue Assay), Alizarin Red-S (AR-s) staining, alkaline phosphatase (ALP) activity, osteocalcin (OCN), and collagen I (Col I) were evaluated. Also, field emission scanning electron microscopy (FESEM) images were acquired in order to visualise the cells and the topography of the material. The proliferation of cells growing in a direct contact with the material was slower at early stages of the study because of the new environmental conditions. However, the entire surface was colonized after 28 days of culture in growth medium (GM). Osteoblastic differentiation markers were significantly enhanced in cells growing on Nurse's A phase ceramic and cultured with osteogenic medium (OM), probably due to the role of silica to stimulate the differentiation of MSCs. Moreover, calcium nodules were formed under the influence of ceramic material. Therefore, it is predicted that Nurse's A-phase ceramic would present high biocompatibility and osteoinductive properties and would be a good candidate to be used as a biomaterial for bone tissue engineering.
本研究的目的是评估一种特性明确的护士A相（7CaO·P₂O₅·2SiO₂）陶瓷的生物活性和细胞反应，并将其与对照物（组织培养聚苯乙烯-TCPS）相比，对体外间充质干细胞（MSCs）的黏附、活力、增殖和成骨分化的影响。评估了细胞增殖（alamar蓝分析法）、茜素红S（AR-s）染色、碱性磷酸酶（ALP）活性、骨钙素（OCN）和I型胶原（Col I）。此外，采集了场发射扫描电子显微镜（FESEM）图像，以便观察细胞和材料的形貌。由于新的环境条件，在研究早期，与材料直接接触生长的细胞增殖较慢。然而，在生长培养基（GM）中培养28天后，整个表面都被细胞定植。在护士A相陶瓷上生长并用成骨培养基（OM）培养的细胞中，成骨分化标志物显著增强，这可能是由于二氧化硅刺激MSCs分化的作用。此外，在陶瓷材料的影响下形成了钙结节。因此，预计护士A相陶瓷将具有高生物相容性和骨诱导特性，并且将是用作骨组织工程生物材料的良好候选者。
