Mei May Lei, Li Quan-Li, Chu Chun Hung
Faculty of Dentistry, The University of Hong Kong, Hong Kong 999077, China.
College of Stomatology, Anhui Medical University, Hefei 230032, China.
Materials (Basel). 2016 May 25;9(6):414. doi: 10.3390/ma9060414.
Root caries prevention has been a challenge for clinicians due to its special anatomical location, which favors the accumulation of dental plaque. Researchers are looking for anti-biofouling material to inhibit bacterial growth on exposed root surfaces. This study aimed to develop polydopamine-induced-polyethylene glycol (PEG) and to study its anti-biofouling effect against a multi-species cariogenic biofilm on the root dentine surface. Hydroxyapatite disks and human dentine blocks were divided into four groups for experiments. They received polydopamine-induced-PEG, PEG, polydopamine, or water application. Contact angle, quartz crystal microbalance, and Fourier transform infrared spectroscopy were used to study the wetting property, surface affinity, and an infrared spectrum; the results indicated that PEG was induced by polydopamine onto a hydroxyapatite disk. Salivary mucin absorption on hydroxyapatite disks with polydopamine-induced-PEG was confirmed using spectrophotometry. The growth of a multi-species cariogenic biofilm on dentine blocks with polydopamine-induced-PEG was assessed and monitored by colony-forming units, confocal laser scanning microscopy, and scanning electron microscopy. The results showed that dentine with polydopamine-induced-PEG had fewer bacteria than other groups. In conclusion, a novel polydopamine-induced-PEG coating was developed. Its anti-biofouling effect inhibited salivary mucin absorption and cariogenic biofilm formation on dentine surface and thus may be used for the prevention of root dentine caries.
由于根龋特殊的解剖位置有利于牙菌斑的积聚，因此对临床医生来说，预防根龋一直是一项挑战。研究人员正在寻找抗生物污染材料，以抑制暴露根面的细菌生长。本研究旨在开发聚多巴胺诱导聚乙二醇（PEG），并研究其对根面牙本质表面多种致龋生物膜的抗生物污染作用。将羟基磷灰石圆盘和人牙本质块分为四组进行实验。它们分别接受聚多巴胺诱导的PEG、PEG、聚多巴胺或水处理。使用接触角、石英晶体微天平以及傅里叶变换红外光谱来研究润湿性、表面亲和力和红外光谱；结果表明，聚多巴胺将PEG诱导到羟基磷灰石圆盘上。使用分光光度法证实了聚多巴胺诱导的PEG在羟基磷灰石圆盘上对唾液粘蛋白的吸附。通过菌落形成单位、共聚焦激光扫描显微镜和扫描电子显微镜对聚多巴胺诱导的PEG在牙本质块上多种致龋生物膜的生长进行评估和监测。结果显示，聚多巴胺诱导的PEG处理的牙本质上的细菌比其他组少。总之，开发了一种新型的聚多巴胺诱导的PEG涂层。其抗生物污染作用抑制了牙本质表面唾液粘蛋白的吸附和致龋生物膜的形成，因此可用于预防根面牙本质龋。
