Ruan Xiao, Yang Li, Cui Wen-Xia, Zhang Men-Xing, Li Zhao-Hui, Liu Ben, Wang Qiang
Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
Materials (Basel). 2016 Jun 29;9(7):524. doi: 10.3390/ma9070524.
Supercritical fluid extraction (SFE) was used to extract total alkaloids, peimisine, peimine and peiminine from the bulb of Miq. The antioxidant capacity of the extracts was evaluated by DPPH radical scavenging activity (DPPH-RSA), ABTS radical scavenging activity (ABTS-RSA) and ferric reducing capacity (FRAP) assay. A central composite design (CCD) with four variables and five levels was employed for optimization of process parameters, and response surface plots were constructed in accordance with a second order polynomial model. Under optimal conditions of 3.0 h, 60.4 °C, 26.5 MPa and 89.3% ethanol, the highest yields were predicted to be 3.8 mg/g for total alkaloids, 0.5 mg/g for peimisine, 1.3 mg/g for peimine and 1.3 mg/g for peiminine, and the antioxidant capacity of extracts displayed EC value of 5.5 mg/mL, EC value of 0.3 mg/mL and FRAP value of 118.2 mg ascorbic acid equivalent (AAE)/100 g.
采用超临界流体萃取(SFE)法从浙贝母鳞茎中提取总生物碱、浙贝宁、浙贝母碱和浙贝甲素。通过DPPH自由基清除活性(DPPH-RSA)、ABTS自由基清除活性(ABTS-RSA)和铁还原能力(FRAP)测定法评估提取物的抗氧化能力。采用具有四个变量和五个水平的中心复合设计(CCD)对工艺参数进行优化,并根据二阶多项式模型构建响应面图。在3.0小时、60.4℃、26.5兆帕和89.3%乙醇的最佳条件下,预测总生物碱的最高产量为3.8毫克/克,浙贝宁为0.5毫克/克,浙贝母碱为1.3毫克/克,浙贝甲素为1.3毫克/克,提取物的抗氧化能力表现为EC值5.5毫克/毫升、EC值0.3毫克/毫升和FRAP值118.2毫克抗坏血酸当量(AAE)/100克。