Chen Lihua, Liu Lili, Zhu Weifeng, Zhang Huimin, Yan Zhihong, Liu Hongning
Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of TCM, Nanchang, China.
Pharmazie. 2011 Sep;66(9):684-9.
A sensitive LC-MS-MS method has been successfully applied to pharmacokinetic study of peimine and peiminine in rat plasma after oral administration of Fritillaria thunbergii Miq. exact and Fritillaria thunbergii Miq. - Glycyrrhiza uralensis Fisch. couple extract. The results indicated that plasma profiles of peimine and peiminine confirmed to two-compartment open model with weighting function of 1/C2 for data fitting and parameter estimation and the utilization with Glycyrrhiza uralensis Fisch. could decrease C(max) and prolong MRT(0-infinity) and t1/2 of peimine remarkly with the bioavailability of peimine remained practically unchanged. Meanwhile, the concentration of peimine in rat plasma was more stable. Nevertheless, there were no significant differences among all calculated parameters of peiminine.
一种灵敏的液相色谱-串联质谱法已成功应用于大鼠口服浙贝母正品及浙贝母-甘草配伍提取物后血浆中贝母碱和去氢贝母碱的药代动力学研究。结果表明，贝母碱和去氢贝母碱的血浆药时曲线符合二室开放模型，采用1/C2加权函数进行数据拟合和参数估算，与甘草配伍使用可显著降低贝母碱的C(max)，延长MRT(0-∞)和t1/2，而贝母碱的生物利用度基本保持不变。同时，大鼠血浆中贝母碱的浓度更稳定。然而，去氢贝母碱所有计算参数之间无显著差异。
