Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, & Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA.
J Biotechnol. 2017 Oct 10;259:91-94. doi: 10.1016/j.jbiotec.2017.07.036. Epub 2017 Jul 31.
n-caproic acid (CA) is a valuable chemical feedstock for various industrial applications. Biological production of CA from renewable carbon sources has attracted a lot of attentions recently. We lately reported the new culture Ruminococcaceae bacterium CPB6, which was isolated from a microbiome for efficient CA production from lactate. To further elucidate its metabolism, we sequenced the whole genome of the strain. The size of the complete genome is 2,069,994bp with 50.58% GC content; no plasmid was identified. Sets of genes involved in the fatty acid biosynthesis via acyl carrier protein (ACP) and coenzyme A (CoA) as well as lactate oxidation/reduction pathways were identified in the genome. These genes were inferred to be correlated with the CA production. The complete genome sequence provides essential information for the elucidation of the metabolism for CA production from lactate, and further improvement of the strain through genetic engineering for enhanced CA production and other biotechnological purposes.
己酸（CA）是一种有价值的化学原料，可用于各种工业应用。最近，人们对利用可再生碳源生物合成 CA 产生了很大的兴趣。我们最近报道了一种新的瘤胃球菌科细菌 CPB6，它是从微生物组中分离出来的，能够高效地从乳酸生产 CA。为了进一步阐明其代谢途径，我们对该菌株进行了全基因组测序。该菌株的全基因组大小为 2069994bp，GC 含量为 50.58%；未鉴定出质粒。基因组中鉴定出了通过酰基辅酶 A（ACP）和辅酶 A（CoA）进行脂肪酸生物合成以及乳酸氧化/还原途径的相关基因。这些基因被推断与 CA 的生产有关。完整的基因组序列为阐明从乳酸生产 CA 的代谢途径提供了必要的信息，并通过遗传工程进一步改进该菌株，以提高 CA 的产量和用于其他生物技术目的。
Zotero 插件