Mansour Abdallah Tag-Eldein, Goda Ashraf Abdelsamee, Omar Eglal Ali, Khalil Hala Saber, Esteban Maria Ángeles
Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt; Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
Aquaculture Division, National Institute of Oceanography and Fisheries, Cairo, Egypt.
Fish Shellfish Immunol. 2017 Sep;68:516-524. doi: 10.1016/j.fsi.2017.07.060. Epub 2017 Jul 31.
Oxidative stress is considered one of the main obstacles to immune competence and high mortality rate of marine fish larvae due to the high dietary polyunsaturated fatty acids and accelerated metabolic rate. This study was carried out to assess the effect of different dietary selenium (Se) yeast on growth, antioxidant status, hematological changes, and cellular and humoral immune parameters in meagre (Argyrosomus regius). Juvenile specimens (3.20 ± 0.17 g) were randomly assigned to four experimental groups, a control group fed a basal diet and another three groups fed Se-supplemented diets at doses of 1, 2 and 3 mg Se-yeast kg diet for 63 days and the final analyzed selenium concentrations were 0.77, 1.51, 2.97 and 3.98 mg Se kg diet, respectively. The results indicated that growth performance, feed conversion ratio and survival were significantly improved with 2.97 and 3.98 mg Se-yeast kg compared to the control group. The catalase, superoxide dismutase activities and total antioxidant status were significantly increased, and thiobarbituric reactive substances in liver homogenate were significantly decreased with increasing Se supplementation respect to the control fish in a dose-dependent manner. Furthermore, hematological and innate immune parameters (immunoglobulin, lysozyme, myeloperoxidase, ACH and respiratory burst activity) were also significantly higher in fish fed the Se-yeast supplemented diets compared to the control group. The results demonstrated that the supplementation of 3.98 mg Se-yeast kg diet improves growth performance, antioxidant balance and innate immune status of meagre juveniles.
由于海洋鱼类幼体的高膳食多不饱和脂肪酸和加速的代谢率，氧化应激被认为是免疫能力的主要障碍之一，也是导致其高死亡率的原因之一。本研究旨在评估不同膳食硒（Se）酵母对欧洲舌齿鲈（Argyrosomus regius）生长、抗氧化状态、血液学变化以及细胞和体液免疫参数的影响。将幼鱼样本（3.20 ± 0.17克）随机分为四个实验组，一个对照组投喂基础饲料，另外三个组分别投喂添加剂量为1、2和3毫克硒酵母/千克饲料的饲料，为期63天，最终分析得到的硒浓度分别为0.77、1.51、2.97和3.98毫克硒/千克饲料。结果表明，与对照组相比，添加2.97和3.98毫克硒酵母/千克饲料可显著提高生长性能、饲料转化率和存活率。随着硒添加量相对于对照鱼的增加，过氧化氢酶、超氧化物歧化酶活性和总抗氧化状态显著增加，肝脏匀浆中的硫代巴比妥酸反应性物质显著减少，呈剂量依赖性。此外，与对照组相比，投喂添加硒酵母饲料的鱼的血液学和先天免疫参数（免疫球蛋白、溶菌酶、髓过氧化物酶、ACH和呼吸爆发活性）也显著更高。结果表明，添加3.98毫克硒酵母/千克饲料可提高欧洲舌齿鲈幼鱼的生长性能、抗氧化平衡和先天免疫状态。
