Mohammad Shafiee Soheil, Amiri Fatemeh, Yousefi Faezeh, Akbari Nasab Negar
Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Medical Laboratory Sciences, School of Paramedicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Rep Biochem Mol Biol. 2024 Jan;12(4):609-618. doi: 10.61186/rbmb.12.4.609.
The oxidant/ antioxidant balance is disrupted in anemia. Antioxidant capacity depends on antioxidant enzyme activity and some trace elements. This study aimed to evaluate oxidant/ antioxidant status and its correlation with red blood cell indices and anemia severity in anemic patients.
Blood samples were taken from 90 anemic patients and 95 healthy people. Circulatory miR-122 was assayed by real-time PCR. Malondialdehyde (MDA), pro-oxidant/ antioxidant balance (PAB), supper oxide demitasse (SOD), glutathione peroxidase (GPxs) activity, total antioxidant capacity (TAC), and zinc were measured by colorimetric method. Selenium was also determined using atomic absorption.
Selenium and zinc decreased significantly in the case group (**P=0.004 and ***P=0.000). The amount of miR-122 up-regulated in the anemia (**P=0.003). MDA was significantly raised in the case control (***P=0.0002). PAB was higher in the case group (**P=0.005). SOD and GPxs activity was decreased along with TAC in anemic patients (*P=0.02, **P=0.008, *P=0.038). Zinc and PAB levels correlated with some red blood cell indices. PAB was associated with anemia severity.
Increased PAB and decreased zinc/selenium increased oxidant levels in anemic patients. RBC indices and anemia severity were correlated with oxidant/ antioxidant somewhere.
贫血患者体内氧化剂/抗氧化剂平衡被打破。抗氧化能力取决于抗氧化酶活性和一些微量元素。本研究旨在评估贫血患者的氧化剂/抗氧化剂状态及其与红细胞指数和贫血严重程度的相关性。
采集90例贫血患者和95例健康人的血样。采用实时聚合酶链反应检测循环中的miR-122。采用比色法测定丙二醛（MDA）、促氧化剂/抗氧化剂平衡（PAB）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPxs）活性、总抗氧化能力（TAC）和锌含量。同时采用原子吸收法测定硒含量。
病例组中硒和锌含量显著降低（**P=0.004，***P=0.000）。贫血患者中miR-122水平上调（**P=0.003）。病例组中MDA显著升高（***P=0.0002）。病例组中PAB较高（**P=0.005）。贫血患者中SOD和GPxs活性以及TAC均降低（*P=0.02，**P=0.008，*P=0.038）。锌和PAB水平与一些红细胞指数相关。PAB与贫血严重程度相关。
贫血患者中PAB升高和锌/硒降低会增加氧化剂水平。红细胞指数和贫血严重程度在一定程度上与氧化剂/抗氧化剂相关。
