Jayappa H G, Lehr C
Am J Vet Res. 1986 Oct;47(10):2217-21.
Pathogenicity evaluations of Moraxella bovis strain EPP 63 grown in the piliated and nonpiliated phase indicated that the organism grown in the piliated phase induced clinical keratoconjunctivitis, whereas the organism grown in the nonpiliated phase did not induce disease under identical challenge conditions. Calves inoculated with culture grown in the piliated phase developed significantly (P less than 0.01) higher lesion scores than did calves inoculated with culture grown in the nonpiliated phase. Vaccination/challenge exposure evaluations indicated that calves immunized with vaccine prepared from piliated culture had significantly lower lesion scores than did control calves (P less than 0.001) or calves immunized with vaccine prepared from nonpiliated culture (P less than 0.05). Similarly, calves immunized with piliated vaccine developed a significantly higher antibody titer against purified pili. Nonvaccinated controls and calves immunized with nonpiliated vaccine did not develop a significant increase in antibody titer. The results indicate that M bovis pili constitute an important factor in the pathogenicity of keratoconjunctivitis and may be used as a vaccine in the control of keratoconjunctivitis in cattle.
对在菌毛形成期和非菌毛形成期生长的牛莫拉菌EPP 63菌株进行的致病性评估表明，在菌毛形成期生长的该菌可诱发临床性角膜结膜炎，而在非菌毛形成期生长的该菌在相同的攻击条件下不会诱发疾病。接种菌毛形成期培养物的犊牛比接种非菌毛形成期培养物的犊牛产生的病变评分显著更高（P小于0.01）。疫苗接种/攻击暴露评估表明，用由菌毛形成期培养物制备的疫苗免疫的犊牛比对照犊牛（P小于0.001）或用由非菌毛形成期培养物制备的疫苗免疫的犊牛（P小于0.05）产生的病变评分显著更低。同样，用菌毛形成期疫苗免疫的犊牛针对纯化菌毛产生的抗体滴度显著更高。未接种疫苗的对照犊牛和用非菌毛形成期疫苗免疫的犊牛的抗体滴度没有显著升高。结果表明，牛莫拉菌菌毛是角膜结膜炎致病性的一个重要因素，可作为控制牛角膜结膜炎的一种疫苗。
