Lepper A W, Hermans L R
Aust Vet J. 1986 Dec;63(12):401-5.
Hyperimmune serums raised in rabbits to purified pili from 9 Australian and 2 American strains of Moraxella bovis from infectious bovine keratoconjunctivitis (IBK) affected herds were used to study the degree of binding between combinations of antigen and antiserum in a conventional enzyme linked immunosorbent assay (ELISA). With the aid of appropriate absorption tests major antigenic differences among pili were found permitting 6 distinct serogroups to be recognised. Further, production of specific antiserums to representative strains of each serogroup in goats facilitated the development of a double antibody sandwich ELISA which could be used to quantitate pilus expression of a given strain of M. bovis, or to differentiate pilus serogroups of 22 strains of M. bovis obtained from a total of 12 Australian herds. Most isolates were found to belong to serogroups designated IV and V. One strain from the United States of America showed total homology with Australian serogroup IV while the other showed some cross-reactivity with serogroups V and VI.
用感染牛传染性角膜结膜炎（IBK）的牛群中9株澳大利亚和2株美国莫拉菌牛源菌株的纯化菌毛免疫家兔制备的超免疫血清，在传统酶联免疫吸附测定（ELISA）中用于研究抗原与抗血清组合之间的结合程度。借助适当的吸收试验，发现菌毛之间存在主要抗原差异，从而识别出6个不同的血清群。此外，在山羊体内制备针对每个血清群代表性菌株的特异性抗血清，有助于开发一种双抗体夹心ELISA，可用于定量给定牛源莫拉菌菌株的菌毛表达，或区分从总共12个澳大利亚牛群中获得的22株牛源莫拉菌的菌毛血清群。大多数分离株属于血清群IV和V。一株来自美国的菌株与澳大利亚血清群IV完全同源，而另一株与血清群V和VI有一些交叉反应。
