Turner Richard, Joseph Adrian, Titchener-Hooker Nigel, Bender Jean
MedImmune LLC Gaithersburg Headquarters, One MedImmune Way, Gaithersburg, MD, 20878, USA.
The Advanced Centre of Biochemical Engineering, Department of Biochemical Engineering, University College London, Bernard Katz Building, London, WC1E 6BT, UK.
Adv Biochem Eng Biotechnol. 2018;165:95-114. doi: 10.1007/10_2016_54.
Cell harvesting is the separation or retention of cells and cellular debris from the supernatant containing the target molecule Selection of harvest method strongly depends on the type of cells, mode of bioreactor operation, process scale, and characteristics of the product and cell culture fluid. Most traditional harvesting methods use some form of filtration, centrifugation, or a combination of both for cell separation and/or retention. Filtration methods include normal flow depth filtration and tangential flow microfiltration. The ability to scale down predictably the selected harvest method helps to ensure successful production and is critical for conducting small-scale characterization studies for confirming parameter targets and ranges. In this chapter we describe centrifugation and depth filtration harvesting methods, share strategies for harvest optimization, present recent developments in centrifugation scale-down models, and review alternative harvesting technologies.
细胞收获是指从含有目标分子的上清液中分离或截留细胞及细胞碎片。收获方法的选择很大程度上取决于细胞类型、生物反应器操作模式、工艺规模以及产品和细胞培养液的特性。大多数传统收获方法采用某种形式的过滤、离心或两者结合来进行细胞分离和/或截留。过滤方法包括常规流深层过滤和切向流微滤。能够可预测地缩小所选收获方法的规模有助于确保生产成功，对于开展小规模表征研究以确定参数目标和范围至关重要。在本章中，我们描述了离心和深层过滤收获方法，分享收获优化策略，介绍离心缩小模型的最新进展，并综述其他收获技术。
