Srivastava Neha, Srivastava Manish, Manikanta Ambepu, Singh Pardeep, Ramteke P W, Mishra P K, Malhotra Bansi D
Department of Chemical Engineering and Technology, Indian Institute of Technology, (BHU), Varanasi, 221005, India.
Department of Physics & Astrophysics, University of Delhi, Delhi, 110007, India.
Appl Biochem Biotechnol. 2017 Oct;183(2):601-612. doi: 10.1007/s12010-017-2561-x. Epub 2017 Aug 3.
Cellulase enzymes have versatile industrial applications. This study was directed towards the isolation, production, and characterization of cellulase enzyme system. Among the five isolated fungal cultures, Emericella variecolor NS3 showed maximum cellulase production using untreated orange peel waste as substrate using solid-state fermentation (SSF). Maximum enzyme production of 31 IU/gds (per gram of dry substrate) was noticed at 6.0 g concentration of orange peel. Further, 50 °C was recorded as the optimum temperature for cellulase activity and the thermal stability for 240 min was observed at this temperature. In addition, the crude enzyme was stable at pH 5.0 and held its complete relative activity in presence of Mn and Fe. This study explored the production of crude enzyme system using biological waste with future potential for research and industrial applications.
纤维素酶具有多种工业应用。本研究旨在分离、生产和表征纤维素酶系统。在五种分离出的真菌培养物中，杂色曲霉NS3以未处理的橙皮废料为底物，采用固态发酵（SSF）显示出最高的纤维素酶产量。在橙皮浓度为6.0克时，观察到最大酶产量为31国际单位/克干底物（每克干底物）。此外，50°C被记录为纤维素酶活性的最佳温度，在此温度下观察到240分钟的热稳定性。此外，粗酶在pH 5.0时稳定，在锰和铁存在下保持其完全相对活性。本研究探索了利用生物废料生产粗酶系统，具有未来研究和工业应用的潜力。
