Forsberg A, Wolf-Watz H
Department of Microbiology, National Defence Research Institute, S-901 82 Umeå, Sweden and Department of Microbiology, University of Umeå, S-907 87 Umeå, Sweden.
Mol Microbiol. 1988 Jan;2(1):121-133. doi: 10.1111/j.1365-2958.1988.tb00013.x.
The DNA sequence of the structural gene (yopE) of one of the Yersinia pseudotuberculosis virulence plas-mid-plB1-encoded proteins, Yop5, is presented. The deduced protein showed a molecular weight of 22971 Daltons. A specific mutant, having a kanamycin-resistance fragment inserted within the yopE gene was no longer virulent for mice. The expression of the Yop5 protein is regulated at the level of transcription by temperature as well as by the Ca -concentration of the medium. A significant increase in the level of transcription was not detected until 45 min after a temperature shift from 26°C to 37°C in the absence of calcium; addition of Ca inhibited the expression. The yopE promoter is under positive, as well as negative, plB1-encoded control. The positive function is solely regulated by temperature, while the regulation of the negative function involves at least five different plasmid-encoded gene loci; one of these genes encodes the V-antigen.
本文展示了假结核耶尔森氏菌毒力质粒pIB1编码的一种蛋白质Yop5的结构基因（yopE）的DNA序列。推导的蛋白质分子量为22971道尔顿。一个在yopE基因内插入了卡那霉素抗性片段的特定突变体对小鼠不再具有毒力。Yop5蛋白的表达在转录水平受温度以及培养基中钙浓度的调节。在无钙条件下，温度从26°C转变为37°C后45分钟之前未检测到转录水平的显著增加；添加钙会抑制表达。yopE启动子受pIB1编码的正调控和负调控。正调控功能仅受温度调节，而负调控功能的调节至少涉及五个不同的质粒编码基因位点；其中一个基因编码V抗原。
