Nakamura Yoshiyuki, Tajima Sigeyuki, Kikuchi Kazuhiro
Saitama Prefectural Agricultural Technology Research Center, Kumagaya, Japan.
Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
Anim Sci J. 2017 Dec;88(12):1916-1924. doi: 10.1111/asj.12855. Epub 2017 Aug 4.
The quality of porcine blastocysts produced in vitro is poor in comparison with those that develop in vivo. We examined the quality of in vitro-matured and fertilized (IVM/IVF) oocytes, their abilities to develop to blastocysts under in vivo and in vitro conditions, and the potential of the embryos to develop to term after transfer. IVM/IVF oocytes were either transferred and the embryos recovered on Days 5 and 6 (100% and 87.5%, respectively) ('ET-vivo' embryos), or cultured in vitro for 5 or 6 days ('IVC' embryos). The proportion of blastocysts differed significantly between the two groups on Day 5 (20.6% and 8.0%, respectively), but not on Day 6 (23.8% and 21.2%, respectively). The mean number of cells in ET-vivo blastocysts on Days 5 or 6 was significantly higher (72.8 and 78.7, respectively) than that in IVC blastocysts (22.1 and 39.7, respectively). When IVM/IVF oocytes and IVC blastocysts on Day 6 were transferred, all (three and three, respectively) developed to piglets (16 and 16, respectively), without any difference in the rates of development to term (2.1% and 2.6%, respectively). These data suggest that, although blastocyst production differs between the two culture conditions, IVM/IVF oocytes possess the same ability to develop to term.
与体内发育的猪囊胚相比，体外产生的猪囊胚质量较差。我们研究了体外成熟和受精（IVM/IVF）卵母细胞的质量、它们在体内和体外条件下发育成囊胚的能力，以及胚胎移植后发育至足月的潜力。IVM/IVF卵母细胞要么进行移植，并在第5天和第6天回收胚胎（分别为100%和87.5%）（“ET-体内”胚胎），要么在体外培养5天或6天（“IVC”胚胎）。两组在第5天的囊胚比例有显著差异（分别为20.6%和8.0%），但在第6天没有差异（分别为23.8%和21.2%）。第5天或第6天ET-体内囊胚的平均细胞数显著高于IVC囊胚（分别为72.8和78.7）（分别为22.1和39.7）。当移植第6天的IVM/IVF卵母细胞和IVC囊胚时，所有（分别为3个和3个）都发育成了仔猪（分别为16头和16头），发育至足月的比率没有差异（分别为2.1%和2.6%）。这些数据表明，尽管两种培养条件下的囊胚产生情况不同，但IVM/IVF卵母细胞具有相同的发育至足月的能力。
