Kaneko Hiroyuki, Kikuchi Kazuhiro, Men Nguyen Thi, Noguchi Junko
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan.
The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
Anim Sci J. 2019 Mar;90(3):344-352. doi: 10.1111/asj.13160. Epub 2019 Jan 17.
Ovarian xenografting makes it possible to obtain oocytes with fertilization ability from immature pigs of Western breeds. In this study, we applied these methods to the Meishan, an indigenous Chinese pig breed, and investigated the developmental competence of oocytes grown in their neonatal tissue after grafting into nude mice. First, mice harboring neonatal ovarian tissue were infused with follicle stimulating hormone (FSH) (62.5 U/ml) for 13 days starting at 10, 30, and 60 days after vaginal opening (D10-, D30-, and D60-FSH groups, respectively). Development of antral follicles and their oocytes was most enhanced in the D60-FSH group. For the next step, we examined the in vitro maturation ability of the oocytes recovered from host mice after infusion with FSH at a dose of 62.5 U/ml or 125 U/ml (FSH-62.5 or -125 group) for 13 days starting at 60 days after vaginal opening. Many more oocytes with maturation ability were obtained from the FSH-125 group. The FSH-125 mature oocytes were fertilized in vitro, as shown by formation of male and female pronuclei, but did not reach the blastocyst stage. These results indicate that Meishan neonatal ovaries are able to produce oocytes with fertilization ability after being grafted into nude mice.
卵巢异种移植使得从西方品种的未成熟猪中获得具有受精能力的卵母细胞成为可能。在本研究中，我们将这些方法应用于中国本土猪种梅山猪，并研究了移植到裸鼠体内后在其新生组织中生长的卵母细胞的发育能力。首先，从阴道开口后10天、30天和60天开始（分别为D10 -、D30 -和D60 - FSH组），向携带新生卵巢组织的小鼠注射促卵泡激素（FSH）（62.5 U/ml），持续13天。D60 - FSH组的窦卵泡及其卵母细胞的发育增强最为明显。下一步，我们检测了从阴道开口后60天开始以62.5 U/ml或125 U/ml的剂量（FSH - 62.5或 - 125组）注射FSH 13天后从宿主小鼠中回收的卵母细胞的体外成熟能力。FSH - 125组获得了更多具有成熟能力的卵母细胞。FSH - 125成熟卵母细胞在体外受精，表现为雄原核和雌原核的形成，但未达到囊胚阶段。这些结果表明，梅山猪新生卵巢移植到裸鼠体内后能够产生具有受精能力的卵母细胞。
