Fitzgerald Michael, Gibbs Chelsea, Shimpi Adrian A, Deans Tara L
Department of Bioengineering, University of Utah , Salt Lake City, Utah 84112, United States.
ACS Synth Biol. 2017 Nov 17;6(11):2014-2020. doi: 10.1021/acssynbio.7b00149. Epub 2017 Aug 14.
The field of mammalian synthetic biology seeks to engineer enabling technologies to create novel approaches for programming cells to probe, perturb, and regulate gene expression with unprecedented precision. To accomplish this, new genetic parts continue to be identified that can be used to build novel genetic circuits to re-engineer cells to perform specific functions. Here, we establish a new transcription-based genetic circuit that combines genes from the quinic acid sensing metabolism of Neorospora crassa and the bacterial Lac repressor system to create a new orthogonal genetic tool to be used in mammalian cells. This work establishes a novel genetic tool, called LacQ, that functions to regulate gene expression in Chinese hamster ovarian (CHO) cells, human embryonic kidney 293 (HEK293) cells, and in mouse embryonic stem (ES) cells.
哺乳动物合成生物学领域致力于开发使能技术，以创造全新方法对细胞进行编程，从而以前所未有的精度探测、扰动和调控基因表达。为实现这一目标，人们不断发现新的遗传元件，可用于构建新型遗传回路，对细胞进行重新设计以执行特定功能。在此，我们构建了一种基于转录的新型遗传回路，它结合了粗糙脉孢菌奎尼酸传感代谢中的基因和细菌乳糖阻遏物系统，从而创建了一种可用于哺乳动物细胞的新型正交遗传工具。这项工作建立了一种名为LacQ的新型遗传工具，它能够在中华仓鼠卵巢（CHO）细胞、人胚肾293（HEK293）细胞以及小鼠胚胎干细胞中调控基因表达。
