Architecture et Fonction des Macromolécules Biologiques, CNRS, Aix-Marseille Université, 13288 Marseille, France.
Emergence des Pathologies Virales (EPV), Aix-Marseille Université, UMR_D 190 IRD French Institute of Research for Development, U1207 INSERM, EHESP French School of Public Health, Marseille, France; IHU Méditerranée Infection, APHM Public Hospital Samsos of Marseille, Marseille, France.
Acta Crystallogr D Struct Biol. 2017 Aug 1;73(Pt 8):650-659. doi: 10.1107/S2059798317008774. Epub 2017 Jul 28.
Toscana virus (TOSV) is an arthropod-borne virus belonging to the Phlebovirus genus within the Bunyaviridae family. As in other bunyaviruses, the genome of TOSV is made up of three RNA segments. They are encapsidated by the nucleoprotein (N), which also plays an essential role in virus replication. To date, crystallographic structures of phlebovirus N have systematically revealed closed-ring organizations which do not fully match the filamentous organization of the ribonucleoprotein (RNP) complex observed by electron microscopy. In order to further bridge the gap between crystallographic data on N and observations of the RNP by electron microscopy, the structural organization of recombinant TOSV N was investigated by an integrative approach combining X-ray diffraction crystallography, transmission electron microscopy, small-angle X-ray scattering, size-exclusion chromatography and multi-angle laser light scattering. It was found that in solution TOSV N forms open oligomers consistent with the encapsidation mechanism of phlebovirus RNA.
托斯卡纳病毒（TOSV）是一种节肢动物传播的病毒，属于布尼亚病毒科的白蛉病毒属。与其他布尼亚病毒一样，TOSV 的基因组由三个 RNA 片段组成。它们被核蛋白（N）包裹，核蛋白在病毒复制中也起着至关重要的作用。迄今为止，白蛉病毒 N 的晶体结构已系统地揭示了封闭环组织，这些结构与电子显微镜观察到的核糖核蛋白（RNP）复合物的丝状组织不完全匹配。为了进一步弥合 N 的晶体学数据与电子显微镜观察到的 RNP 之间的差距，通过结合 X 射线衍射晶体学、透射电子显微镜、小角度 X 射线散射、尺寸排阻色谱和多角度激光光散射的综合方法，研究了重组 TOSV N 的结构组织。结果发现，在溶液中，TOSV N 形成与白蛉病毒 RNA 包装机制一致的开放寡聚物。
