Nanomedicine Lab, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, M13 9PT, UK.
Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, M13 9PT, UK.
Adv Healthc Mater. 2017 Nov;6(21). doi: 10.1002/adhm.201700374. Epub 2017 Aug 4.
Direct labeling of human mesenchymal stem cells (hMSC) prior to transplantation provides a means to track cells after administration and it is a powerful tool for the assessment of new cell-based therapies. Biocompatible nanoprobes consisting of liposome-indocyanine green hybrid vesicles (liposome-ICG) are used to safely label hMSC. Labeled hMSC recapitulating a 3D cellular environment is transplanted as spheroids subcutaneously and intracranially in athymic nude mice. Cells emit a strong NIR signal used for tracking post-transplantation with the IVIS imaging system up to 2 weeks (subcutaneous) and 1 week (intracranial). The transplanted stem cells are imaged in situ after engraftment deep in the brain up to 1 week in living animals using optical imaging techniques and without the need to genetically modify the cells. This method is proposed for efficient, nontoxic direct cell labeling for the preclinical assessment of cell-based therapies and the design of clinical trials, and potentially for localization of the cell engraftment after transplantation into patients.
在移植前对人间质干细胞（hMSC）进行直接标记为细胞给药后的追踪提供了一种手段，也是评估新型细胞疗法的有力工具。由脂质体-吲哚菁绿杂化囊泡（liposome-ICG）组成的生物相容性纳米探针被用于安全标记 hMSC。标记后的 hMSC 以球体形式在无胸腺裸鼠的皮下和颅内进行移植，再现了 3D 细胞环境。细胞发出强烈的近红外信号，可使用 IVIS 成像系统在移植后进行长达 2 周（皮下）和 1 周（颅内）的跟踪。使用光学成像技术，在活体动物中，在大脑深部植入后可对移植的干细胞进行原位成像，无需对细胞进行基因修饰。该方法拟用于细胞疗法的临床前评估和临床试验的高效、无毒的直接细胞标记，并有可能用于移植后患者的细胞植入定位。
