Lorig-Roach Nicholas, Still Patrick C, Coppage David, Compton Jennifer E, Crews Mitchell S, Navarro Gabriel, Tenney Karen, Crews Phillip
Department of Chemistry and Biochemistry, University of California , Santa Cruz, California 95064, United States.
J Nat Prod. 2017 Aug 25;80(8):2304-2310. doi: 10.1021/acs.jnatprod.7b00302. Epub 2017 Aug 4.
The biosynthetic potential of marine-sediment-derived Gram-negative bacteria is poorly understood. Sampling of California near-shore marine environments afforded isolation of numerous Gram-negative bacteria in the Proteobacteria and Bacteriodetes phyla, which were grown in the laboratory to provide extracts whose metabolites were identified by comparative analyses of LC-mass spectrometry and MS data. Overall, we developed an assemblage of seven bacterial strains grown in five different media types designed to coax out unique secondary metabolite production as a function of varying culture conditions. The changes in metabolite production patterns were tracked using the GNPS MS fragmentation pattern analysis tool. A variety of nitrogen-rich metabolites were visualized from the different strains grown in different media, and strikingly, all of the strains examined produced the same new, proton-atom-deficient compound, 1-methyl-4-methylthio-β-carboline (1), CHNS. Scale-up liquid culture of Achromobacter spanius (order: Burkholderiales; class: Betaproteobacteria) provided material for the final structure elucidation. The methods successfully combined in this work should stimulate future studies of molecules from marine-derived Gram-negative bacteria.
人们对源自海洋沉积物的革兰氏阴性菌的生物合成潜力了解甚少。对加利福尼亚近岸海洋环境进行采样，分离出了变形菌门和拟杆菌门中的众多革兰氏阴性菌，在实验室中培养这些细菌以提供提取物，通过液相色谱 - 质谱联用和质谱数据的比较分析来鉴定其代谢产物。总体而言，我们培养了一组七种细菌菌株，它们生长在五种不同的培养基类型中，旨在根据不同的培养条件诱导出独特的次生代谢产物。使用GNPS质谱碎片模式分析工具追踪代谢产物产生模式的变化。从在不同培养基中生长的不同菌株中观察到了多种富含氮的代谢产物，而且引人注目的是，所有检测的菌株都产生了相同的新的、缺质子原子的化合物1 - 甲基 - 4 - 甲硫基 - β - 咔啉（1），CHNS。扩展培养西班牙无色杆菌（目：伯克霍尔德氏菌目；纲：β - 变形菌纲）为最终的结构解析提供了材料。这项工作中成功结合的方法应该会推动未来对源自海洋的革兰氏阴性菌分子的研究。
