CIEPQPF, Department of Chemical Engineering, Universidade de Coimbra, P-3030 790 Coimbra, Portugal.
CICS-UBI, Health Sciences Research Center, Universidade da Beira Interior, P-6200 506 Covilhã, Portugal.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:7-15. doi: 10.1016/j.colsurfb.2017.07.065. Epub 2017 Jul 26.
Coaxial electrospinning is a technique that allows the production of nanofibers with a core-shell structure. Such fibers present several advantages as materials for the preparation of scaffolds, namely due to the possibility of combining a core with the desired mechanical properties with a shell prepared from biocompatible materials that will establish proper interactions with the host. Herein, core-shell fibrous meshes, composed of a polycaprolactone (PCL) core and a functionalized gelatin shell, were prepared by coaxial electrospinning and then photocrosslinked under UV light aiming to be used in vascular tissue regeneration. The suitability of the meshes for the pretended biomedical application was evaluated by assessing their chemical/physical properties as well as their haemo and biocompatibility in vitro. The obtained results revealed that meshes' shell prepared with a higher content of gelatin showed fibers with diameters presenting a unimodal distribution and a mean value of 600nm. Moreover, those fibers with higher content of gelatin also displayed lower water contact angles, and therefore higher hydrophilicities. Such features are crucial for the good biologic performance displayed by these meshes, when in contact with blood and with Normal Human Dermal Fibroblasts cells.
同轴静电纺丝是一种能够制备核壳结构纳米纤维的技术。这种纤维作为支架材料具有许多优点，因为它可以将具有所需机械性能的核与由生物相容性材料制成的壳结合起来，壳与宿主之间可以建立适当的相互作用。本文通过同轴静电纺丝制备了由聚己内酯（PCL）核和功能化明胶壳组成的核壳纤维网，然后在紫外光下光交联，旨在用于血管组织再生。通过评估其化学/物理性质以及体外血液相容性和生物相容性，评估了该纤维网在预期的生物医学应用中的适用性。结果表明，用较高含量的明胶制备的纤维网的壳具有直径呈单峰分布且平均值为 600nm 的纤维。此外，那些含有较高含量明胶的纤维还表现出较低的水接触角，因此具有更高的亲水性。当这些纤维网与血液和正常人皮肤成纤维细胞接触时，这些特性对于它们良好的生物学性能至关重要。
