Correia T R, Ferreira P, Vaz R, Alves P, Figueiredo M M, Correia I J, Coimbra P
CICS-UBI, Health Sciences Research Center, University of Beira Interior, P-6200 506 Covilhã, Portugal.
CIEPQPF, Department of Chemical Engineering, University of Coimbra, P-3030 790 Coimbra, Portugal.
Int J Biol Macromol. 2016 Dec;93(Pt B):1539-1548. doi: 10.1016/j.ijbiomac.2016.05.045. Epub 2016 May 13.
Cardiovascular disease is the leading cause of morbidity and mortality among industrialized countries. Vascular grafts are often required for the surgical treatments. Considering the limitations associated with the use of autografts and with the currently available synthetic materials, a growing demand in tissue engineered vascular grafts has been registered. During the work here described, electrospinning technique was used to prepared fibrous matrices to be applied as vascular implants. For that purpose, electrospun polycaprolactone (PCL) fibrous mats were produced and afterwards coated with different hydrogel formulations based in photocrosslinkable gelatin (GelMA) and the macromers poly(ethylene glycol) acrylate (PEGA) and poly(ethylene glycol) diacrylate (PEGDA). These were further photocrosslinked under UV irradiation using Irgacure 2959 (by BASF) as the photoinitiator. The suitability of the coated scaffolds for the intended application, was evaluated by assessing their chemical/physical properties as well as their interaction with blood and endothelial cells.
心血管疾病是工业化国家发病和死亡的主要原因。手术治疗通常需要血管移植物。考虑到自体移植物和目前可用合成材料使用的局限性，组织工程血管移植物的需求日益增加。在本文所述的工作中，采用静电纺丝技术制备用作血管植入物的纤维基质。为此，制备了静电纺聚己内酯（PCL）纤维垫，然后用基于可光交联明胶（GelMA）以及大分子单体聚（乙二醇）丙烯酸酯（PEGA）和聚（乙二醇）二丙烯酸酯（PEGDA）的不同水凝胶配方进行涂层。使用巴斯夫公司的Irgacure 2959作为光引发剂，在紫外线照射下对这些涂层进行进一步的光交联。通过评估其化学/物理性质以及它们与血液和内皮细胞的相互作用，来评价涂层支架对于预期应用的适用性。
