Pertseva M N, Kuznetzova L A, Plesneva S A, Tyurin V A, Gorbunov N V
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1986;85(1):95-101. doi: 10.1016/0742-8413(86)90057-5.
Reconstitution of catecholamine-sensitive adenylate cyclase from chick embryonic muscle membranes and guanyl nucleotide-binding proteins of mature rabbit muscle makes it possible to reveal the coupling (potentiating) effect of these nucleotides 1 week earlier than in the native condition. The effective insertion of guanyl-nucleotide-binding proteins into the embryonic membrane coincides with the onset of a pronounced increase in membrane lipid fluidity during the course of embryogenesis. The different ontogenetic time-courses for the origination of the two guanyl nucleotide effects, on catalytic adenylate cyclase activity (in early embryogenesis) and on the coupling process (in postembryonic life), suggest the existence in this system of two separate guanyl-nucleotide-binding proteins performing regulatory and coupling functions, respectively.
从鸡胚肌肉膜和成熟兔肌肉的鸟苷酸结合蛋白中重构儿茶酚胺敏感的腺苷酸环化酶，使得在比天然状态早1周的时间揭示这些核苷酸的偶联（增强）效应成为可能。鸟苷酸结合蛋白有效插入胚胎膜与胚胎发育过程中膜脂质流动性显著增加的开始时间一致。两种鸟苷酸效应产生的个体发生时间进程不同，一种对催化腺苷酸环化酶活性的效应（在胚胎发育早期），另一种对偶联过程的效应（在胚胎后期），这表明该系统中存在两种分别执行调节和偶联功能的独立鸟苷酸结合蛋白。
