Taebipour Mohammad Jafar, Dadras Habibollah, Nazifi Saeed, Afsar Mina, Ansari-Lari Maryam
Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz 71441-69155, Iran.
Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz 71441-69155, Iran.
Vet Immunol Immunopathol. 2017 Aug;190:31-38. doi: 10.1016/j.vetimm.2017.07.002. Epub 2017 Jul 9.
In the present study, after vaccination and challenge with Newcastle disease virus, changes in the population of blood monocytes and lymphocytes of broiler chickens were evaluated using flow cytometry. 300 apparently healthy 1-day-old Cobb broiler chicks were divided randomly into four experimental groups (n=75). At 20days of age the chicks in group 1 and 2 were vaccinated with live B1 ND vaccine. Those in group 2 were additionally injected with a killed vaccine simultaneously and group 3 chicks received only the adjuvant of the killed vaccine. The birds in groups 1, 2 and 3 were challenged with a velogenic ND virus and those in group 4 were treated as control. Sampling was done on days 1,2,3,7 after vaccination and also on 1, 2, 3,7,14, 21 post challenge days. In this study percentage of B cell population was increased after vaccination and challenge in vaccinated birds, but CD3+ cells were decreased after vaccination and challenge, which showed B cells have more expansion than T cells. The CD4+ cell percentage in vaccinated birds was always lower than control birds. However, the percentage of CD8+ cells in vaccinated birds was increased. Results indicate increased CMI with the live NDV vaccination. In this study CD4/CD8 ratio in control birds was about 1.5 at 30days of age and it was slightly lower in vaccinated and challenged birds. The percentage of monocytes in vaccinated birds was significantly higher than control birds from 3days post vaccination to the end of the experiment.
在本研究中，用新城疫病毒进行疫苗接种和攻毒后，使用流式细胞术评估了肉鸡血液单核细胞和淋巴细胞群体的变化。300只1日龄外观健康的科宝肉鸡随机分为四个实验组（n = 75）。在20日龄时，第1组和第2组的雏鸡用活B1新城疫疫苗进行接种。第2组同时额外注射了灭活疫苗，第3组雏鸡只接受了灭活疫苗的佐剂。第1、2和3组的鸡用强毒新城疫病毒进行攻毒，第4组作为对照。在接种后第1、2、3、7天以及攻毒后第1、2、3、7、14、21天进行采样。在本研究中，接种疫苗的鸡在接种和攻毒后B细胞群体百分比增加，但接种和攻毒后CD3 +细胞减少，这表明B细胞比T细胞有更多的扩增。接种疫苗的鸡中CD4 +细胞百分比始终低于对照鸡。然而，接种疫苗的鸡中CD8 +细胞百分比增加。结果表明活新城疫病毒疫苗接种后细胞介导免疫增加。在本研究中，对照鸡在30日龄时CD4/CD8比值约为1.5，在接种疫苗和攻毒的鸡中略低。从接种后第3天到实验结束，接种疫苗的鸡中单核细胞百分比显著高于对照鸡。
