Ganapathy K, Cox W J, Gough R E, Cargill P, Montiel E, Jones R C
Department of Veterinary Pathology, University of Liverpool, Neston, South Wirral, UK.
Avian Pathol. 2007 Aug;36(4):313-7. doi: 10.1080/03079450701460781.
This paper describes two experiments. In each experiment, 1-day-old specific pathogen free chicks were divided into three groups. In Experiment 1 - [avian metapneumo virus (aMPV) challenge] - one group served as unvaccinated controls; the second group was vaccinated with live aMPV (subtype B) vaccine only, and the third group received the aMPV vaccine in combination with live Newcastle disease virus (NDV) vaccine (VG/GA strain). Oropharyngeal swabs, tissues and blood samples were collected before and after challenge with a virulent subtype aMPV at 21 days post vaccination. Chicks were monitored for post-challenge clinical signs. Swabs and tissues were examined for the detection of challenge aMPV by virus isolation and by reverse-transcriptase polymerase-chain reaction. Sera were assayed for antibodies against aMPV and NDV. The single and combined vaccinated chicks were all protected against clinical signs and no challenge virus was isolated from either of the vaccinated-challenged groups. In Experiment 2 (NDV challenge), as in Experiment 1, chicks were divided into three groups where one group remained as unvaccinated control and the other two groups were vaccinated as above, except that the second group received live NDV vaccine only, instead of aMPV. At 21 days post vaccination, 15 chicks from each of the three groups were removed to a different site and challenged with a virulent NDV (Texas GB strain). Re-isolation of the challenge virus was not attempted. All chicks in both NDV-vaccinated challenged groups were protected against clinical signs and mortality. These results show that, based on parameters monitored for the respective challenge virus, simultaneous application of live aMPV and NDV vaccines did not affect the efficacy of either vaccine.
本文描述了两项实验。在每项实验中，将1日龄的无特定病原体雏鸡分为三组。在实验1 - [禽偏肺病毒（aMPV）攻毒] - 一组作为未接种疫苗的对照；第二组仅接种活的aMPV（B亚型）疫苗，第三组接种aMPV疫苗并联合活的新城疫病毒（NDV）疫苗（VG/GA株）。在接种疫苗后21天用强毒aMPV亚型攻毒前后采集口咽拭子、组织和血液样本。监测雏鸡攻毒后的临床症状。通过病毒分离和逆转录聚合酶链反应检测拭子和组织中攻毒的aMPV。检测血清中抗aMPV和NDV的抗体。单独接种和联合接种的雏鸡均受到保护，未出现临床症状，且在接种疫苗并攻毒的两组中均未分离到攻毒病毒。在实验2（NDV攻毒）中，与实验1一样，雏鸡分为三组，一组作为未接种疫苗的对照，另外两组如上所述接种疫苗，不同的是第二组仅接种活的NDV疫苗，而非aMPV。在接种疫苗后21天，将三组中的每组15只雏鸡转移到不同地点，并用强毒NDV（德克萨斯GB株）攻毒。未尝试重新分离攻毒病毒。接种NDV疫苗并攻毒的两组中的所有雏鸡均受到保护，未出现临床症状和死亡。这些结果表明，根据针对各自攻毒病毒监测的参数，同时应用活的aMPV和NDV疫苗不会影响任何一种疫苗的效力。
