Domínguez-Rodríguez Gloria, Marina María Luisa, Plaza Merichel
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, Alcalá de Henares, Madrid, Spain.
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, Universidad de Alcalá, Ctra. Madrid-Barcelona Km. 33.600, Alcalá de Henares, Madrid, Spain.
J Chromatogr A. 2017 Sep 8;1514:1-15. doi: 10.1016/j.chroma.2017.07.066. Epub 2017 Jul 22.
The majority of studies based on phenolic compounds from plants are focused on the extractable fraction derived from an aqueous or aqueous-organic extraction. However, an important fraction of polyphenols is ignored due to the fact that they remain retained in the residue of extraction. They are the so-called non-extractable polyphenols (NEPs) which are high molecular weight polymeric polyphenols or individual low molecular weight phenolics associated to macromolecules. The scarce information available about NEPs shows that these compounds possess interesting biological activities. That is why the interest about the study of these compounds has been increasing in the last years. Furthermore, the extraction and characterization of NEPs are considered a challenge because the developed analytical methodologies present some limitations. Thus, the present literature review summarizes current knowledge of NEPs and the different methodologies for the extraction of these compounds, with a particular focus on hydrolysis treatments. Besides, this review provides information on the most recent developments in the purification, separation, identification and quantification of NEPs from plants.
大多数基于植物酚类化合物的研究都集中在水相或水-有机萃取得到的可萃取部分。然而，由于多酚的一个重要部分保留在萃取残渣中，因此这部分被忽视了。它们就是所谓的不可萃取多酚（NEPs），是高分子量的聚合多酚或与大分子相关的低分子量酚类单体。关于NEPs的现有信息稀缺，表明这些化合物具有有趣的生物活性。这就是为什么近年来对这些化合物的研究兴趣不断增加。此外，NEPs的提取和表征被认为是一项挑战，因为现有的分析方法存在一些局限性。因此，本文献综述总结了NEPs的现有知识以及这些化合物的不同提取方法，特别关注水解处理。此外，本综述还提供了从植物中纯化、分离、鉴定和定量NEPs的最新进展信息。
