Universidad de Alcalá, Departamento de Química Analítica, Química Física e Ingeniería Química, Facultad de Ciencias, Ctra, Madrid-Barcelona Km 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Universidad de Alcalá, Instituto de Investigación Química Andrés M. del Río (IQAR), Ctra, Madrid-Barcelona Km 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Nutrients. 2021 Sep 17;13(9):3242. doi: 10.3390/nu13093242.
Sweet cherry generates large amounts of by-products within which pomace can be a source of bioactive phenolic compounds. Commonly, phenolic compounds have been obtained by conventional extraction methodologies. However, a significant fraction, called non-extractable polyphenols (NEPs), stays held in the conventional extraction residues. Therefore, in the present work, the release of NEPs from cherry pomace using pressurized liquid extraction (PLE) combined with enzyme-assisted extraction (EAE) using Promod enzyme is investigated for the first time. In order to study the influence of temperature, time, and pH on the NEPs extraction, a response surface methodology was carried out. PLE-EAE extracts displayed higher TPC (75 ± 8 mg GAE/100 g sample) as well as, PA content, and antioxidant capacity than the extracts obtained by PLE (with a TPC value of 14 ± 1 mg GAE/100 g sample) under the same extraction conditions, and those obtained by conventional methods (TPC of 8.30 ± 0.05 mg GAE/100 g sample). Thus, PLE-EAE treatment was more selective and sustainable to release NEPs from sweet cherry pomace compared with PLE without EAE treatment. Besides, size-exclusion chromatography profiles showed that PLE-EAE allowed obtaining NEPs with higher molecular weight (>8000 Da) than PLE alone.
甜樱桃在其果渣中会产生大量的副产物，而这些副产物可能是生物活性酚类化合物的来源。通常，酚类化合物是通过传统的提取方法获得的。然而，仍有相当一部分酚类化合物（称为不可提取多酚，NEPs）残留在传统的提取残渣中。因此，在本研究中，首次采用加压液体萃取（PLE）与 Promod 酶辅助酶解提取（EAE）相结合的方法，从樱桃果渣中释放 NEPs。为了研究温度、时间和 pH 值对 NEPs 提取的影响，采用响应面法进行了研究。与 PLE（TPC 值为 14±1mgGAE/100g 样品）在相同提取条件下，以及与传统方法（TPC 值为 8.30±0.05mgGAE/100g 样品）相比，PLE-EAE 提取物具有更高的 TPC（75±8mgGAE/100g 样品）、PA 含量和抗氧化能力。因此，与没有 EAE 处理的 PLE 相比，PLE-EAE 处理更具选择性和可持续性，可从甜樱桃果渣中释放 NEPs。此外，排阻色谱分析表明，PLE-EAE 可以获得比 PLE 更高分子量（>8000Da）的 NEPs。
