Ullrich A, Gray A, Tam A W, Yang-Feng T, Tsubokawa M, Collins C, Henzel W, Le Bon T, Kathuria S, Chen E
EMBO J. 1986 Oct;5(10):2503-12. doi: 10.1002/j.1460-2075.1986.tb04528.x.
To identify structural characteristics of the closely related cell surface receptors for insulin and IGF-I that define their distinct physiological roles, we determined the complete primary structure of the human IGF-I receptor from cloned cDNA. The deduced sequence predicts a 1367 amino acid receptor precursor, including a 30-residue signal peptide, which is removed during translocation of the nascent polypeptide chain. The 1337 residue, unmodified proreceptor polypeptide has a predicted Mr of 151,869, which compares with the 180,000 Mr IGF-I receptor precursor. In analogy with the 152,784 Mr insulin receptor precursor, cleavage of the Arg-Lys-Arg-Arg sequence at position 707 of the IGF-I receptor precursor will generate alpha (80,423 Mr) and beta (70,866 Mr) subunits, which compare with approximately 135,000 Mr (alpha) and 90,000 Mr (beta) fully glycosylated subunits.
为了确定胰岛素和IGF-I密切相关的细胞表面受体的结构特征，这些特征决定了它们独特的生理作用，我们从克隆的cDNA中确定了人IGF-I受体的完整一级结构。推导的序列预测有一个1367个氨基酸的受体前体，包括一个30个残基的信号肽，在新生多肽链转运过程中被去除。1337个残基的未修饰的前受体多肽预测的Mr为151,869，与180,000 Mr的IGF-I受体前体相比。与152,784 Mr的胰岛素受体前体类似，IGF-I受体前体第707位的Arg-Lys-Arg-Arg序列的切割将产生α（80,423 Mr）和β（70,866 Mr）亚基，与约135,000 Mr（α）和90,000 Mr（β）的完全糖基化亚基相比。
