Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Institute of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Department of Clinical Laboratory Medicine, People's Hospital of Dongxihu District Wuhan City, Wuhan 430040, China.
Exp Gerontol. 2017 Oct 15;97:64-67. doi: 10.1016/j.exger.2017.07.018. Epub 2017 Aug 1.
Previous experiments have demonstrated that several inflammatory biomarkers, including pentraxin 3 (PTX3), matrix metalloprotein 9 (MMP9), interleukin-6 (IL-6), and the neutrophil to lymphocyte ratio (NLR), are differentially elevated in coronary artery disease (CAD). This study aims to compare the associations between plasma levels of these biomarkers and CAD, identifying the best biomarker that has the most powerful association with CAD.
Blood samples were collected from 64 patients admitted to the Department of Cardiology, 31 of whom had CAD and 33 of whom were CAD-free. Plasma levels of PTX3, MMP9, and IL-6 were measured via ELISA. The coronary Gensini score was used to evaluate the severity of coronary artery lesions.
PTX3 levels and NLR levels between the CAD group and the CAD-free group were statistically significant (P<0.05). Stepwise multiple linear regression analysis showed that PTX3 levels and NLR levels were independently associated with CAD (r=1.3, P<0.05; r=1.8, P<0.05). Only PTX3 was associated with the severity of coronary artery stenosis. A PTX3 threshold of 4.38ng/mL maximized true-positive and false-negative results. PTX3 displayed a greater area under the receiver operating characteristic curve (AUC) than NLR, MMP9, and IL-6 (0.733 versus 0.612 versus 0.725 versus 0.518).
Compared to NLR, MMP9, and IL-6, PTX3 displayed greater AUC and association with CAD. PTX3 may become a potentially powerful inflammatory biomarker for CAD.
先前的实验已经证明，几种炎症生物标志物，包括五聚素 3（PTX3）、基质金属蛋白酶 9（MMP9）、白细胞介素 6（IL-6）和中性粒细胞与淋巴细胞比值（NLR），在冠状动脉疾病（CAD）中存在差异升高。本研究旨在比较这些生物标志物与 CAD 之间的关联，确定与 CAD 关联最强的最佳生物标志物。
采集心内科收治的 64 例患者的血样，其中 31 例患有 CAD，33 例无 CAD。通过 ELISA 测量 PTX3、MMP9 和 IL-6 的血浆水平。使用冠状动脉 Gensini 评分评估冠状动脉病变的严重程度。
CAD 组和 CAD 组之间的 PTX3 水平和 NLR 水平有统计学意义（P<0.05）。逐步多元线性回归分析表明，PTX3 水平和 NLR 水平与 CAD 独立相关（r=1.3，P<0.05；r=1.8，P<0.05）。只有 PTX3 与冠状动脉狭窄的严重程度相关。PTX3 阈值为 4.38ng/mL 时，真阳性和假阴性结果最大。PTX3 显示出比 NLR、MMP9 和 IL-6 更大的接受者操作特征曲线下面积（AUC）（0.733 对 0.612 对 0.725 对 0.518）。
与 NLR、MMP9 和 IL-6 相比，PTX3 显示出更大的 AUC 和与 CAD 的关联。PTX3 可能成为 CAD 潜在的强大炎症生物标志物。
