Plisetskaya E M, Pollock H G, Rouse J B, Hamilton J W, Kimmel J R, Andrews P C, Gorbman A
Gen Comp Endocrinol. 1986 Aug;63(2):252-63. doi: 10.1016/0016-6480(86)90163-2.
Three different somatostatins have been isolated from the pancreatic islet tissue of the coho salmon (Oncorhynchus kisutch) by gel filtration and HPLC. Two of these peptides contain 14 amino acids and the larger third peptide consists of 25 amino acids. The sequence of the salmon SST-25 is Ser-Val-Asp-Asn-Leu-Pro-Pro-Arg-Glu-Arg-Lys-Ala-Gly -Cys-Lys-Asn-Phe-Tyr-Trp-Lys-Gly-Phe-Thr-Ser-Cys. The sequence of the salmon SST-14-I is Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys. The other small somatostatin (SST-14-II) which was not sequenced has an amino acid composition identical to the C-terminal 14 amino acids of the SST-25 and it is probably derived from this larger form. Evidence for low levels of a somatostatin containing 28 amino acids is also presented. This SST-28 appears to be an N-terminal extended precursor of SST-25 or a peptide derived via alternative processing of a common preprosomatostatin. Injected into juvenile salmon, SST-25 caused a decline in circulating levels of plasma insulin, depletion of liver glycogen, and activation of lipolytic pathways. Juvenile salmon treated with anti-SST-25 serum revealed elevated levels of plasma insulin as well as an increase of the glycogen content of the liver.
通过凝胶过滤和高效液相色谱法，已从银大麻哈鱼（Oncorhynchus kisutch）的胰岛组织中分离出三种不同的生长抑素。其中两种肽含有14个氨基酸，第三种较大的肽由25个氨基酸组成。鲑鱼SST - 25的序列为Ser - Val - Asp - Asn - Leu - Pro - Pro - Arg - Glu - Arg - Lys - Ala - Gly - Cys - Lys - Asn - Phe - Tyr - Trp - Lys - Gly - Phe - Thr - Ser - Cys。鲑鱼SST - 14 - I的序列为Ala - Gly - Cys - Lys - Asn - Phe - Phe - Trp - Lys - Thr - Phe - Thr - Ser - Cys。另一种未测序的小生长抑素（SST - 14 - II）的氨基酸组成与SST - 25的C末端14个氨基酸相同，它可能来源于这种较大的形式。文中还提供了含有28个氨基酸的生长抑素水平较低的证据。这种SST - 28似乎是SST - 25的N末端延伸前体，或者是通过共同的前生长抑素原的替代加工产生的一种肽。给幼鲑注射SST - 25会导致血浆胰岛素循环水平下降、肝糖原消耗以及脂解途径激活。用抗SST - 25血清处理的幼鲑显示血浆胰岛素水平升高以及肝脏糖原含量增加。
