Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR China; Hubei Engineering Laboratory for Synthetic Microbiology, Wuhan Institute of Biotechnology, Wuhan 430075, PR China.
Curr Opin Biotechnol. 2017 Dec;48:234-241. doi: 10.1016/j.copbio.2017.07.002. Epub 2017 Aug 2.
Terpenoids comprise the largest family of natural products and have widespread applications. The overproduction of value-added terpenoids and the efficient mining of new terpenoids represent major challenges in the fields of metabolic engineering and natural product discovery. For terpenoid overproduction in microbial systems, in vitro reconstitution strategy guided rational engineering was emphasized, and -omics studies provide further information for targeted engineering. In addition, systematic engineering, including host and pathway engineering for terpenoid overproduction was reviewed. Furthermore, robust precursor supplies with optimized high-production hosts can also be used as an efficient platform to rapidly screen terpene cyclases and accelerate the process of mining new terpenoids.
萜类化合物是最大的天然产物家族，具有广泛的应用。在代谢工程和天然产物发现领域，高附加值萜类化合物的过量生产和新型萜类化合物的高效挖掘是主要挑战。对于微生物系统中的萜类化合物过量生产，强调了体外重组策略指导的合理工程，而组学研究为靶向工程提供了进一步的信息。此外，还综述了萜类化合物过量生产的系统工程，包括宿主和途径工程。此外，具有优化高产宿主的稳健前体供应也可以用作快速筛选萜烯合酶和加速挖掘新型萜类化合物的有效平台。
