Identification and differentiation of beta-blockers and their metabolites in urine by computerized gas chromatography-mass spectrometry.

A method for the identification and differentiation of beta-blockers and their metabolites in urine after acid hydrolysis is described. The acetylated extract is analysed by computerized gas chromatography-mass spectrometry. An on-line computer allows rapid detection using ion chromatography with the ions of m/z 72, 86, 98, 140, 151, 159, 200 and 335. The identity of positive signals in the reconstructed ion chromatogram is confirmed by a comparison of the stored entire mass spectra with the reference spectra. The ion chromatogram, the reference mass spectra and the gas chromatographic retention indices (OV-101) are documented. References for the quantitation of the single beta-blockers are cited.