利用计算机化气相色谱-质谱联用技术鉴定和区分尿液中的苯二氮䓬类药物及其代谢物。

Identification and differentiation of benzodiazepines and their metabolites in urine by computerized gas chromatography-mass spectrometry.

作者信息

Maurer H, Pfleger K

机构信息

Institut für Pharmakologie und Toxikologie, Universität des Saarlandes, Homburg/Saar, F.R.G.

出版信息

J Chromatogr. 1987 Nov 27;422:85-101. doi: 10.1016/0378-4347(87)80442-5.

DOI:10.1016/0378-4347(87)80442-5

PMID:3437028

Abstract

A method for the identification and differentiation of the following benzodiazepines and their metabolites in urine after acid hydrolysis and acetylation is described: bromazepam, camazepam, chlordiazepoxide, clobazam, clonazepam, clorazepate, clotiazepam, cloxazolam, delorazepam, diazepam, ethylloflazepate, flunitrazepam, flurazepam, halazepam, ketazolam, loprazolam, lorazepam, lormetazepam, medazepam, metaclazepam, midazolam, nitrazepam, nordazepam, oxazepam, oxazolam, prazepam, quazepam, temazepam and tetrazepam. The acetylated extract was analysed by computerized gas chromatography-mass spectrometry. An on-line computer allowed rapid detection using ion chromatography with ions m/z 205, 211, 230, 241, 245, 249, 312 and 333. The identity of positive signals in the reconstructed ion chromatogram was confirmed by a comparison of the stored full mass spectra with reference spectra. The ion chromatograms, reference mass spectra and gas chromatographic retention indices (on OV-101) are documented.

摘要

本文描述了一种在尿液经酸水解和乙酰化后鉴定和区分以下苯二氮䓬类药物及其代谢物的方法：溴西泮、卡马西泮、氯氮䓬、氯巴占、氯硝西泮、氯氮䓬二钾、氯噻西泮、氯恶唑仑、地洛西泮、地西泮、依替唑仑、氟硝西泮、氟西泮、哈拉西泮、凯他唑仑、洛拉佐仑、劳拉西泮、氯美扎酮、美达西泮、甲氯西泮、咪达唑仑、硝西泮、去甲西泮、奥沙西泮、恶唑仑、普拉西泮、夸西泮、替马西泮和四氮唑仑。乙酰化提取物通过计算机化气相色谱 - 质谱联用仪进行分析。在线计算机通过离子色谱法，利用离子m/z 205、211、230、241、245、249、312和333实现快速检测。通过将存储的全质谱与参考光谱进行比较，确认重建离子色谱图中阳性信号的身份。记录了离子色谱图、参考质谱和气相色谱保留指数（在OV - 101上）。