Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering, Huaiyin Normal University, Huai'an 223300, China.
Jiangsu Province Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huai'an 223300, China.
Bioresour Technol. 2017 Nov;244(Pt 1):289-295. doi: 10.1016/j.biortech.2017.07.148. Epub 2017 Jul 27.
Poly(β-l-malic acid) (PMA) production by Aureobasidium pullulans HA-4D was carried out through solid-state fermentation (SSF) using agro-industrial residues. Maximum PMA production (75.4mg/g substrate) was obtained from a mixed substrate of sweet potato residue and wheat bran (1:1, w/w) supplemented with NaNO (0.8%, w/w) and CaCO (2%, w/w), with an initial moisture content of 70% and inoculum size of 13% (v/w) for 8days. Repeated-batch SSF was successfully conducted for 5 cycles with a high productivity. The scanning electron microscopy showed that the yeast-like cells of A. pullulans HA-4D could grow well on the solid substrate surface. Moreover, the cost analysis showed that the unit price of PMA in SSF was much lower than that of SmF. This is the first report on PMA production via SSF, and this study provided a new method to produce PMA from inexpensive agro-industrial residues.
聚β-苹果酸（PMA）的生产由出芽短梗霉 HA-4D 通过固态发酵（SSF）利用农业工业废料进行。通过使用甘薯渣和麦麸（1:1，w/w）的混合底物，并添加 NaNO（0.8%，w/w）和 CaCO（2%，w/w），在初始含水量为 70%和接种量为 13%（v/w）的条件下发酵 8 天，获得了最大的 PMA 产量（75.4mg/g 底物）。通过重复批次 SSF 成功进行了 5 个周期的生产，具有较高的生产力。扫描电子显微镜显示，出芽短梗霉 HA-4D 的酵母样细胞可以很好地在固体基质表面生长。此外，成本分析表明 SSF 中 PMA 的单价远低于 SmF。这是 SSF 生产 PMA 的首次报道，本研究为利用廉价的农业工业废料生产 PMA 提供了一种新方法。
