Xia Jun, Liu Sili, Jiao Jiali, Qiu Zhongyang, Liu Xiaoyang, He Aiyong, Xu Ning, Xu Jiaxing
Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering, Huaiyin Normal University, Huai'an, 223300, China.
Bioprocess Biosyst Eng. 2022 Oct;45(10):1673-1682. doi: 10.1007/s00449-022-02772-2. Epub 2022 Aug 14.
Polymalic acid (PMA) is a water-soluble polyester produced by Aureobasidium pullulans. In this study, the physiological response of A. pullulans after the addition of vegetable oils was investigated. Soybean oil (SBO) is pivotal for shortening fermentation time and achieving high PMA titer. With the addition of 1% (w/v) SBO, the titer and productivity of PMA was, respectively, increased by 34.2% and 80%. SBO acted as a chemical stimulatory agent rather than a carbon source, the enhancement on PMA production was attributed to the component of fatty acid. SBO induced the dimorphism (yeast-like cells and mycelia) of A. pullulans, in vitro enzyme activities indicated that the TCA oxidative branch for malic acid synthesis might be strengthened, which could generate more ATP for PMA synthesis, and the assay of intracellular energy supply validated this deduction. This study provided a new sight for recognizing the regulatory behavior of SBO in A. pullulans.
聚苹果酸（PMA）是一种由出芽短梗霉产生的水溶性聚酯。在本研究中，研究了添加植物油后出芽短梗霉的生理反应。大豆油（SBO）对于缩短发酵时间和实现高PMA产量至关重要。添加1%（w/v）的SBO后，PMA的产量和生产率分别提高了34.2%和80%。SBO作为一种化学刺激剂而非碳源，其对PMA产量的提高归因于脂肪酸成分。SBO诱导了出芽短梗霉的二态性（酵母样细胞和菌丝体），体外酶活性表明，苹果酸合成的三羧酸循环氧化分支可能得到加强，这可为PMA合成产生更多ATP，细胞内能量供应分析证实了这一推断。本研究为认识SBO在出芽短梗霉中的调控行为提供了新的视角。
