Zhang P P, Li Z J, Wang D Q
Department of Integrated TCM & Western Medicine, Tianjin First Center Hospital, Tianjin 300192, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2017 May 20;35(5):369-372. doi: 10.3760/cma.j.issn.1001-9391.2017.05.014.
To investigate the effect of Liangge powder on the expression of inflammatory mediators and hepatic and renal function in rats with sepsis via a preliminary analysis. A total of 195 Wistar rats were randomly divided into sham-operation group (15 rats) , model group (90 rats) , and treatment group (90 rats) using a random number table, and according to the time point after modeling, the model group and the treatment group were further divided into 24, 48, and 72 hour subgroups, with 30 rats in each subgroup. Cecal ligation and puncture (CLP) was performed to establish a rat model of sepsis, and solid-phase sandwich enzyme-linked immunosorbent assay was used to measure the expression of interleukin-10 (IL-10) , interleukin-1 (IL-1) , interleukin-4 (IL-4) , and lipopolysaccharide (LPS) at 24, 48, and 72 hours after CLP. An automatic biochemical analyzer was used to measure the levels of alanine aminotransferase (ALT) , aspartate aminotransferase (AST) , blood urea nitrogen (BUN) , and creatinine (Cr) . Compared with the sham-operation group, the model group had significant increases in the serum levels of IL-1, IL-4, IL-10, LPS, BUN, Cr, AST, and ALT at 24, 48, and 72 hours after CLP (<0.05) . Compared with the model group, the treatment group had a significant reduction in the serum level of IL-1 at 24, 48, and 72 hours after CLP, a significant increase in the serum level of IL-4 at 24 and 48 hours after CLP, and a significant increase in the serum level of IL-10 and a significant reduction in the serum level of LPS at 24 and 72 hours after CLP (<0.05) . Compared with the model group, the treatment group had a significant reduction in ALT at 24 hours after CLP, significant reductions in BUN and Cr at 48 hours after CLP, and a significant reduction in AST at 72 hours after CLP (<0.05) . In the early stage of sepsis, imbalance between pro-inflammation and anti-inflammation and immunosuppression may cause hepatic and renal impairment in rats with sepsis. The traditional Chinese medicine Liangge powder can regulate inflammatory response, restore the balance between pro-inflammation and anti-inflammation, and improve hepatic and renal function in rats with sepsis.
通过初步分析探讨凉膈散对脓毒症大鼠炎症介质表达及肝肾功能的影响。将195只Wistar大鼠采用随机数字表法随机分为假手术组（15只）、模型组（90只）和治疗组（90只），模型组和治疗组根据建模后时间点进一步分为24、48和72小时亚组，每组30只。采用盲肠结扎穿孔术（CLP）建立大鼠脓毒症模型，采用固相夹心酶联免疫吸附测定法检测CLP术后24、48和72小时白细胞介素-10（IL-10）、白细胞介素-1（IL-1）、白细胞介素-4（IL-4）和脂多糖（LPS）的表达。采用自动生化分析仪检测丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、血尿素氮（BUN）和肌酐（Cr）水平。与假手术组比较，模型组CLP术后24、48和72小时血清IL-1、IL-4、IL-10、LPS、BUN、Cr、AST和ALT水平显著升高（<0.05）。与模型组比较，治疗组CLP术后24、48和72小时血清IL-1水平显著降低，CLP术后24和48小时血清IL-4水平显著升高，CLP术后24和72小时血清IL-10水平显著升高、LPS水平显著降低（<0.05）。与模型组比较，治疗组CLP术后24小时ALT显著降低，CLP术后48小时BUN和Cr显著降低，CLP术后72小时AST显著降低（<0.05）。在脓毒症早期，促炎与抗炎失衡及免疫抑制可能导致脓毒症大鼠肝肾功能损害。中药凉膈散可调节炎症反应，恢复促炎与抗炎平衡，改善脓毒症大鼠肝肾功能。
