Lawrence K E, Forsyth S F, Vaatstra B L, McFadden Amj, Pulford D J, Govindaraju K, Pomroy W E
a Institute of Veterinary, Animal and Biomedical Sciences , Massey University , Private Bag 11222, Palmerston North , 4442 , New Zealand.
b Gribbles Veterinary Pathology , 840 Tremaine Ave, Palmerston North , 4412 , New Zealand.
N Z Vet J. 2017 Nov;65(6):305-312. doi: 10.1080/00480169.2017.1364180. Epub 2017 Aug 21.
AIM To determine the most commonly used words in the clinical histories of animals naturally infected with Theileria orientalis Ikeda type; whether these words differed between cases categorised by age, farm type or haematocrit (HCT), and if there was any clustering of the common words in relation to these categories. METHODS Clinical histories were transcribed for 605 cases of bovine anaemia associated with T. orientalis (TABA), that were submitted to laboratories with blood samples which tested positive for T. orientalis Ikeda type infection by PCR analysis, between October 2012 and November 2014. χ tests were used to determine whether the proportion of submissions for each word was similar across the categories of HCT (normal, moderate anaemia or severe anaemia), farm type (dairy or beef) and age (young or old). Correspondence analysis (CA) was carried out on a contingency table of the frequency of the 28 most commonly used history words, cross-tabulated by age categories (young, old or unknown). Agglomerative hierarchical clustering, using Ward's method, was then performed on the coordinates from the correspondence analysis. RESULTS The six most commonly used history words were jaundice (204/605), lethargic (162/605), pale mucous membranes (161/605), cow (151/605), anaemia (147/605), and off milk (115/605). The proportion of cases with some history words differed between categories of age, farm type and HCT. The cluster analysis indicated that the recorded history words were grouped in two main clusters. The first included the words weight loss, tachycardia, pale mucous membranes, anaemia, lethargic and thin, and was associated with adult (p<0.001), severe anaemia (p<0.001) and dairy (p<0.001). The second cluster included the words deaths, ill-thrift, calves, calf and diarrhoea, and was associated with young (p<0.001), normal HCT (p<0.001), beef (p<0.001) and moderate anaemia (p<0.001). CONCLUSIONS AND CLINICAL RELEVANCE Cluster analysis of words recorded in clinical histories submitted with blood samples from cases of TABA indicates that two potentially different disease syndromes were associated with T. orientalis Ikeda type infection. One was consistent with the affected cattle suffering from a severe regenerative extravascular haemolytic anaemia, the second displaying as ill thrift and diarrhoea, particularly in young beef cattle.
目的 确定自然感染东方泰勒虫池田株的动物临床病史中最常用的词汇；这些词汇在按年龄、农场类型或血细胞比容（HCT）分类的病例之间是否存在差异，以及这些常用词汇在这些类别中是否存在聚类情况。方法 转录了605例与东方泰勒虫相关的牛贫血（TABA）病例的临床病史，这些病例于2012年10月至2014年11月期间提交至实验室，其血液样本经聚合酶链反应（PCR）分析检测出东方泰勒虫池田株感染呈阳性。采用χ检验来确定每个词汇在HCT类别（正常、中度贫血或重度贫血）、农场类型（奶牛场或肉牛场）和年龄（年轻或年老）中的提交比例是否相似。对28个最常用病史词汇的频率列联表进行对应分析（CA），该列联表按年龄类别（年轻、年老或未知）交叉制表。然后使用Ward方法对对应分析的坐标进行凝聚层次聚类。结果 六个最常用的病史词汇是黄疸（204/605）、嗜睡（162/605）、黏膜苍白（161/605）、母牛（151/605）、贫血（147/605）和停奶（115/605）。某些病史词汇的病例比例在年龄、农场类型和HCT类别之间存在差异。聚类分析表明，记录的病史词汇主要分为两个聚类。第一个聚类包括体重减轻、心动过速、黏膜苍白、贫血、嗜睡和消瘦等词汇，与成年牛（p<0.001）、重度贫血（p<0.001）和奶牛场（p<0.001）相关。第二个聚类包括死亡、生长不良、犊牛、小牛和腹泻等词汇，与年轻牛（p<0.001）、正常HCT（p<0.001）、肉牛场（p<0.001）和中度贫血（p<0.001）相关。结论与临床意义 对TABA病例血液样本所附临床病史中记录的词汇进行聚类分析表明，两种潜在不同的疾病综合征与东方泰勒虫池田株感染相关。一种与受影响的牛患有严重的再生性血管外溶血性贫血一致，另一种表现为生长不良和腹泻，特别是在年轻肉牛中。
