日本一日龄肉鸡雏鸡中产CTX-M-25肠杆菌科细菌的出现情况。
The occurrence of CTX-M-25-producing Enterobacteriaceae in day-old broiler chicks in Japan.
作者信息
Yossapol Montira, Sugiyama Michiyo, Asai Tetsuo
机构信息
Department of Applied Veterinary Science, the United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Office of Academic Affairs, Faculty of Veterinary Sciences, Mahasarakham University, Talad sub-district, Mueang district, Maha Sarakham 44000, Thailand.
出版信息
J Vet Med Sci. 2017 Oct 7;79(10):1644-1647. doi: 10.1292/jvms.17-0294. Epub 2017 Aug 7.
Day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between January and July 2016. Sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant Enterobacteriaceae. Cefotaxime (CTX)-resistant Klebsiella pneumoniae (1 isolate) and Enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. Conjugation experiments revealed that the bla gene conferring CTX resistance was transferred from the K. pneumoniae isolate and 2 of the 4 E. cloacae isolates to Escherichia coli DH5α via IncA/C plasmids carrying the gene. Our results suggested that the bla gene originating from chicks may be spread among commercial broiler farms.
2016年1月至7月期间，从3个孵化场选取一日龄雏鸡，放置在垫纸上，然后运至一家商业肉鸡养殖场。收集了66份被雏鸡胎粪污染的垫纸样本，用于检测头孢菌素耐药肠杆菌科细菌的分离情况。从66份样本中的5份（7.58%）分离出了耐头孢噻肟（CTX）的肺炎克雷伯菌（1株）和阴沟肠杆菌（4株）。接合实验表明，携带CTX耐药bla基因的IncA/C质粒将赋予CTX耐药性的bla基因从肺炎克雷伯菌分离株以及4株阴沟肠杆菌分离株中的2株转移至大肠杆菌DH5α。我们的结果表明，源自雏鸡的bla基因可能会在商业肉鸡养殖场中传播。
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