Fang Lei, Li Jialian, Zhou Jie, Wang Xiao, Guo Lanping
School of Biological Science and Technology, University of Jinan, Jinan 250022, China.
Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan 250014, China.
Molecules. 2017 Aug 7;22(8):1310. doi: 10.3390/molecules22081310.
High-speed counter-current chromatography was used to separate and purify ecdysteroids for the first time from the stems of using a two-phase solvent system composed of ethyl acetate--butanol-ethanol-water (3:0.2:0.8:3, /). Three ecdysteroids were obtained from 260 mg of ethyl acetate extract of the residue obtained after evaporation of the crude ethanolicextractof in one-step separation, which were identified as paristerone (, 30.5 mg), ecdysterone (, 7.2 mg), and capitasterone (, 8.1 mg) by electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR). Their anti-inflammatory activities were evaluated by measuring the inhibitory ratios of β-glucuronidase release in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor. Compounds - showed significant anti-inflammatory activities with IC-values ranging from 1.51 to 11.68 μM, respectively.
首次采用高速逆流色谱法，以乙酸乙酯 - 丁醇 - 乙醇 - 水（3:0.2:0.8:3，v/v）组成的两相溶剂系统，从[植物名称未给出]的茎中分离纯化蜕皮甾体。在一步分离中，从粗乙醇提取物蒸发后得到的残渣的260 mg乙酸乙酯提取物中获得了三种蜕皮甾体，通过电喷雾电离质谱（ESI-MS）和核磁共振（NMR）鉴定为孕甾酮（[含量未给出]，30.5 mg）、蜕皮甾酮（[含量未给出]，7.2 mg）和头花甾酮（[含量未给出]，8.1 mg）。通过测量血小板活化因子诱导的大鼠多形核白细胞（PMN）中β - 葡萄糖醛酸酶释放的抑制率来评估它们的抗炎活性。化合物[未明确具体化合物编号]显示出显著的抗炎活性，IC50值分别为1.51至11.68 μM。
