Okekunle Akinkunmi Paul, Li Ying, Liu Liyan, Du Shanshan, Wu Xiaoyan, Chen Yang, Li Yanchuan, Qi Jiayue, Sun Changhao, Feng Rennan
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin, Heilongjiang Province 150081, People's Republic of China.
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 157 Baojian Road, Nangang District, Harbin, Heilongjiang Province 150081, People's Republic of China.
Diabetes Res Clin Pract. 2017 Oct;132:45-58. doi: 10.1016/j.diabres.2017.07.023. Epub 2017 Jul 21.
To evaluate circulating amino acids (AA) profiles in obesity, type 2 diabetes (T2D) and metabolic syndrome (MetS).
Serum AA were profiled among 200; healthy, obese, T2D and MetS subjects matched by sex, age and BMI using ultra-high performance liquid chromatography tandem quadruple mass spectrometry (UPLC-TQ-MS). A meta-analysis, including 47 case-control studies (including the current study) on serum AA in obesity, T2D and MetS searched through October 2016 was conducted to explore the AA differences in obesity, T2D and MetS.
In comparison with healthy controls, 14 AA (10 increased and 4 decreased) were significantly altered (P<0.05) in all non-healthy subjects. Also, mean differences of valine (obese: 34.13 [27.70, 40.56]µmol/L, P<0.001, T2D: 19.49 [3.31, 35.68]µmol/L, P<0.05, MetS: 29.18 [16.04, 42.33]µmol/L, P<0.001), glutamic acid (obese: 18.62 [11.64, 25.61]µmol/L, P<0.001, T2D: 19.94 [0.28, 39.61]µmol/L, P<0.05, MetS: 12.45 [3.98, 20.91]µmol/L, P<0.001), proline (obese: 16.72 [6.20, 27.24]µmol/L, P<0.001, T2D: 20.72 [15.82, 25.61]µmol/L, P<0.001, MetS: 29.95 [25.18, 34.71]µmol/L, P<0.001) and isoleucine (obese: 11.39 [8.54, 14.24]µmol/L, P<0.001, T2D: 7.37 [1.52, 13.22]µmol/L, P<0.05, MetS: 10.40 [4.90, 15.89]µmol/L, P<0.001) were significantly higher compared to healthy controls. Similarly, mean differences of glycine (obese: -30.99 [-39.69, -22.29]µmol/L, P<0.001, T2D: -30.37 [-41.80, -18.94]µmol/L, P<0.001 and MetS: -35.24 [-39.28, -31.21]µmol/L, P<0.001) were significantly lower compared to healthy controls.
In both the case-control study and meta-analysis, obesity was related to the most circulating AA changes, followed by MetS and T2D. Valine, isoleucine, glutamic acid and proline increased, while Glycine decreased in all metabolic disorders.
评估肥胖、2型糖尿病（T2D）和代谢综合征（MetS）患者的循环氨基酸（AA）谱。
采用超高效液相色谱串联四极杆质谱法（UPLC-TQ-MS），对200名按性别、年龄和体重指数（BMI）匹配的健康、肥胖、T2D和MetS受试者的血清AA进行分析。通过检索截至2016年10月的47项关于肥胖、T2D和MetS患者血清AA的病例对照研究（包括本研究）进行荟萃分析，以探讨肥胖、T2D和MetS患者AA的差异。
与健康对照组相比，所有非健康受试者中14种AA（10种升高，4种降低）有显著变化（P<0.05）。此外，缬氨酸（肥胖组：34.13[27.70,40.56]µmol/L，P<0.001；T2D组：19.49[3.31,35.68]µmol/L，P<0.05；MetS组：29.18[16.04,42.33]µmol/L，P<0.001）、谷氨酸（肥胖组：18.62[11.64,25.61]µmol/L，P<0.001；T2D组：19.94[0.28,39.61]µmol/L，P<0.05；MetS组：12.45[3.98,20.91]µmol/L，P<0.001）、脯氨酸（肥胖组：16.72[6.20,27.24]µmol/L，P<0.001；T2D组：20.72[15.82,25.61]µmol/L，P<0.001；MetS组：29.95[25.18,34.71]µmol/L，P<0.001）和异亮氨酸（肥胖组：11.39[8.54,14.24]µmol/L，P<0.001；T2D组：7.37[1.52,13.22]µmol/L，P<0.05；MetS组：10.40[4.90,15.89]µmol/L，P<0.001）的平均差异与健康对照组相比显著升高。同样，甘氨酸的平均差异（肥胖组：-30.99[-39.69,-22.29]µmol/L，P<0.001；T2D组：-30.37[-41.80,-18.94]µmol/L，P<0.001；MetS组：-35.24[-39.28,-31.21]µmol/L，P<0.001）与健康对照组相比显著降低。
在病例对照研究和荟萃分析中，肥胖与循环AA变化最多相关，其次是MetS和T2D。在所有代谢紊乱中，缬氨酸、异亮氨酸、谷氨酸和脯氨酸升高，而甘氨酸降低。
