Maltais-Payette Ina, Lajeunesse-Trempe Fannie, Nadeau Mélanie, Bouvet-Bouchard Léonie, Hould Frédéric Simon, Biertho Laurent, Tchernof André
Heart and Lung Institute, Laval University, Quebec, QC G1V 4G5, Canada.
School of Nutrition, Faculty of Agricultural and Food Sciences, Laval University, Quebec, QC G1V 0A6, Canada.
Metabolites. 2025 Apr 30;15(5):297. doi: 10.3390/metabo15050297.
Studies using metabolomics to study bariatric surgery have shown that amino acids are one of the most changed groups of metabolites after the intervention. However, the surgery-related variation in individual amino acids, as well as the long-term impact and the differences between the types of surgeries, have been poorly studied. The aim of this study was to investigate the changes in circulating amino acids after three types of bariatric surgery up to 36 months after the intervention.
We studied 63 participants diagnosed with T2D at baseline, who received either a sleeve gastrectomy, a Roux-en-Y gastric bypass or a biliopancreatic diversion with duodenal switch. We measured the concentrations of 16 circulating amino acids in fasting plasma before the surgery as well as after 4, 12, 24 and 36 months via liquid chromatography coupled with mass spectrometry (LC-MS/MS).
Eleven circulating amino acids were significantly modified by bariatric surgery. Glutamate, leucine and isoleucine showed the greatest decrease. Most of the changes in circulating amino acids occurred within 1 year of the operations. Only one measured plasmatic amino acid (threonine) had a significantly different change pattern according to surgery types. In repeated-measure correlations, changes in circulating amino acids were significantly associated with changes in adiposity and metabolic markers.
Bariatric surgery changes the levels of most circulating amino acids, and the effect occurs in the short term without major differences between surgery types. The mechanisms explaining these changes are not elucidated but likely include modifications in amino acid metabolism.
利用代谢组学研究减肥手术的研究表明，氨基酸是干预后代谢物变化最大的类别之一。然而，个体氨基酸的手术相关变化，以及长期影响和手术类型之间的差异，尚未得到充分研究。本研究的目的是调查三种减肥手术后长达36个月循环氨基酸的变化。
我们研究了63名基线诊断为2型糖尿病的参与者，他们接受了袖状胃切除术、Roux-en-Y胃旁路术或胆胰转流十二指肠转位术。我们通过液相色谱-质谱联用（LC-MS/MS）测量了手术前以及术后4、12、24和36个月空腹血浆中16种循环氨基酸的浓度。
减肥手术显著改变了11种循环氨基酸。谷氨酸、亮氨酸和异亮氨酸下降最为明显。循环氨基酸的大多数变化发生在手术后1年内。根据手术类型，只有一种测量的血浆氨基酸（苏氨酸）有显著不同的变化模式。在重复测量相关性分析中，循环氨基酸的变化与肥胖和代谢标志物的变化显著相关。
减肥手术改变了大多数循环氨基酸的水平，且这种影响在短期内出现，不同手术类型之间无重大差异。解释这些变化的机制尚未阐明，但可能包括氨基酸代谢的改变。
