Stelletta Calogero, Tekin Koray, Tirpan Mehmet Borga, Alemdar Havva, Cil Beste, Oztutar Stelletta Fatma, Olgac Kemal Tuna, Inanc Muhammed Enes, Daskin Ali
Department of Animal Medicine, Production and Health, University of Padova, Legnaro PD, Italy; Department of Animal Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Department of Animal Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
Theriogenology. 2017 Nov;103:137-142. doi: 10.1016/j.theriogenology.2017.07.038. Epub 2017 Jul 27.
Aim of this study was to study the vulvar thermal pattern variation during the timed artificial insemination protocol in Angora goat and identify the relationship with the successful rate. Does (36 adult healthy females) were synchronized using PGF2α at the day 0, 11 days of progesterone impregnated sponges intra-vaginally, PMSG 48 h before sponges withdraw (day 11) and the intra-cervical inseminations were carried out 48 h later (Timed Artificial Insemination: TAI) with chilled semen. Vulvar (VST) and perivulvar (PST) areas were considered to evaluate the thermal pattern during the protocol at the day 0 and at the TAI using a thermo camera (E60, FLIR System). Differences of temperature (ΔT) between the surfaces were calculated for each time. The does were monitored for pregnancy, delivery time and prolificacy. Pregnant (P) and non-pregnant (NP) does were compared in terms of VST, PST and ΔT using two ways ANOVA considering time and pregnancy as sources of variability. VST was lower than PST in all the monitored does (P < 0.05) (34.79 ± 0.14 vs 36.58 ± 0.14 °C) and without differences between P and NP at day 0 (35 ± 0.18 vs 36.39 ± 0.22 °C). Significant difference (P < 0.05) between P and NP does was recorded at TAI in terms of VST (33.89 ± 0.31 vs 35.40 ± 0.24 °C) and ΔT (-3.16 ± 0.34 vs -1.62 ± 0.26 °C). In conclusion thermal emission by glabrous surfaces in goat may be used to identify the right response induced by hormonal treatments and to optimize the application of assisted reproductive techniques at the field level.
本研究的目的是研究安哥拉山羊定时人工授精方案期间外阴温度模式的变化，并确定其与成功率的关系。母羊（36只成年健康雌性）在第0天使用PGF2α进行同期发情处理，在第11天经阴道放置含孕酮的海绵栓，在取出海绵栓前48小时（第11天）注射PMSG，并在48小时后（定时人工授精：TAI）用冷冻精液进行子宫颈内授精。在第0天和TAI时，使用热成像仪（E60，FLIR系统）评估外阴（VST）和外阴周围（PST）区域的温度模式，计算每次表面之间的温度差（ΔT）。监测母羊的妊娠情况、分娩时间和繁殖力。使用双因素方差分析，将时间和妊娠作为变异来源，比较怀孕（P）和未怀孕（NP）母羊的VST、PST和ΔT。在所有监测的母羊中，VST低于PST（P < 0.05）（34.79 ± 0.14 vs 36.58 ± 0.14°C），在第0天P和NP之间无差异（35 ± 0.18 vs 36.39 ± 0.22°C）。在TAI时，P和NP母羊在VST（33.89 ± 0.31 vs 35.40 ± 0.24°C）和ΔT（-3.16 ± 0.34 vs -1.62 ± 0.26°C）方面存在显著差异（P < 0.05）。总之，山羊无毛表面的热发射可用于识别激素处理引起的正确反应，并在现场水平优化辅助生殖技术的应用。
