Kim Kwan-Woo, Lee Jinwook, Kim Keun Jung, Lee Eun-Do, Kim Sung Woo, Lee Sung-Soo, Lee Sang-Hoon
Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea.
Institute of Chungnam Livestock Research, Cheongyang 33350, Korea.
J Anim Sci Technol. 2021 Jan;63(1):36-45. doi: 10.5187/jast.2021.e10. Epub 2021 Jan 31.
Presently, there is an increased demand for livestock products all over the world which has led to more devotion on improving livestock population. Although goats have been bred for a long time in Korea, but there is not much research conducted on traditional Korean black goat () compared to other livestock populations. Mutton consumption has been dramatically changing from medicinal use to edible meat and this trend directs the black goat populations declining and also mutton import quantities are increasing consistently. The present study introduced a new estrus synchronizing technique with subsequent artificial insemination (AI) for Korean black goats to enable crossbreeding with non-native breeds for the small or subsistent farmers. Our data highlighted that, the percentage of motile sperm from the electro-ejaculated samples declined significantly after freezing and melting. In addition, the sperm motility significantly declined with regard to sperm incubation period (0, 5, 60, and 120 min at 37°C) and was negatively correlated (64.2 ± 7.9%, 63.3 ± 5.8%, 49.9 ± 6.3%, and 35.9 ± 7.6%, respectively) in frozen-thawed sperm samples. Moreover, the E2 levels were unchanged even 24 h after controlled internal drug releas (CIDR) withdrawal. But, 48 h and 72 h after CIDR removal, E2 levels increased significantly. These data helps us to consider the two time points for AI; CIDR removal after 24 h, at which E2 decreases, and after 48 h, as the time at which progesterone increases. Additionally, the AI after 48 h of CIDR removal group exhibited significantly higher pregnancy and parturition rates (42.9%) compared to AI after 24 h after CIDR removal 28.6% group. In conclusion, these studies will propose an optimal estrus synchronisation process with subsequent timing of AI and also will promote the Korean black goat breeding industry.
目前，全球对畜产品的需求不断增加，这使得人们更加致力于提高牲畜数量。尽管韩国养殖山羊已有很长时间，但与其他牲畜群体相比，对韩国传统黑山羊（）的研究并不多。羊肉消费已从药用急剧转变为食用肉，这种趋势导致黑山羊数量减少，羊肉进口量也在持续增加。本研究为韩国黑山羊引入了一种新的发情同步技术及后续人工授精（AI）方法，以使小农户或自给型农户能够与非本地品种进行杂交育种。我们的数据表明，电刺激采精样本经冷冻和解冻后，活动精子的百分比显著下降。此外，精子活力在精子孵育期（37°C下0、5、60和120分钟）显著下降，在冻融精子样本中呈负相关（分别为64.2±7.9%、63.3±5.8%、49.9±6.3%和35.9±7.6%）。此外，即使在取出控制内部药物释放（CIDR）装置24小时后，雌二醇（E2）水平也没有变化。但是，在取出CIDR装置48小时和72小时后，E2水平显著升高。这些数据有助于我们考虑人工授精的两个时间点：取出CIDR装置24小时后，此时E2下降；48小时后，此时孕酮增加。此外，与取出CIDR装置24小时后进行人工授精的28.6%的组相比，取出CIDR装置48小时后进行人工授精的组的妊娠率和分娩率显著更高（42.9%）。总之，这些研究将提出一个最佳的发情同步过程及后续人工授精的时间安排，也将推动韩国黑山羊养殖业的发展。
