Rodrigues Maira da Silva, Siqueira-Silva Diógenes Henrique de, Quirino Patrícia Postingel, Ninhaus-Silveira Alexandre, Veríssimo-Silveira Rosicleire
UNESP - Univ. Estadual Paulista, Faculdade de Engenharia de Ilha Solteira, Departamento de Biologia e Zootecnia, L.I.NEO - Laboratório de Ictiologia Neotropical. Av: Brasil, Centro, No 56. Ilha Solteira, 15385000, São Paulo, Brazil.
UNIFESSPA - UNIVERSIDADE FEDERAL DO SUL E SUDESTE DO PARÁ - Instituto de estudos em Saúde e Biologicas - IESB, Faculdade de Ciências da Saúde e Biologicas - FACISB, Folha 31, Quadra 07, Lote especial s/n - Nova Marabá - PA, CEP 68507-590, Brazil.
Theriogenology. 2017 Nov;103:149-152. doi: 10.1016/j.theriogenology.2017.07.025. Epub 2017 Jul 27.
This stereological analysis of the types of germ cells and the number of Sertoli cells per cyst in Astyanax altiparanae testes during spermatogenesis is the first such report in Characiformes. Testes of 25 male A. altiparanae were examined. Based on the number of spermatogonia B per cyst (469.2 ± 9.92), we estimated that spermatogonia undergo at least nine mitotic divisions before differentiating into primary spermatocytes. There are four spermatogonia types: undifferentiated spermatogonia A*, undifferentiated spermatogonia, differentiated spermatogonia, and type B spermatogonia. The number of Sertoli cells increased gradually from 1.41 ± 0.51 in the single undifferentiated spermatogonium A* to 9.25 ± 0.50 in cysts of spermatocytes in the leptotene/zygotene stage, possibly related to greater complexity of cellular events during the meiotic stage. The number of germ cells rose dramatically from spermatogonia A (1.0 ± 0) to spermatogonia B (469.2 ± 9.92); however, the quantity of spermatocytes inside the cysts in the leptotene/zygotene stage decreased (300.6 ± 6.97) relative to spermatogonia B, representing a loss of approximately 36% of the former number of cells. This was probably the result of apoptosis, which promotes successful development of the remaining cells during sperm production. The support capacity of Sertoli cells increased gradually during spermatogenesis.
对高身丽脂鲤精子发生过程中生殖细胞类型和每个精小囊支持细胞数量进行的这种体视学分析，是脂鲤目鱼类中的首份此类报告。对25条雄性高身丽脂鲤的睾丸进行了检查。根据每个精小囊B型精原细胞的数量（469.2±9.92），我们估计精原细胞在分化为初级精母细胞之前至少经历了9次有丝分裂。有四种精原细胞类型：未分化的A型精原细胞、未分化的精原细胞、分化型精原细胞和B型精原细胞。支持细胞的数量从单个未分化的A型精原细胞中的1.41±0.51逐渐增加到细线期/偶线期精母细胞精小囊中的9.25±0.50，这可能与减数分裂阶段细胞事件的更大复杂性有关。生殖细胞的数量从A型精原细胞（1.0±0）急剧增加到B型精原细胞（469.2±9.92）；然而，细线期/偶线期精小囊内的精母细胞数量相对于B型精原细胞减少了（300.6±6.97），相当于之前细胞数量减少了约36%。这可能是细胞凋亡的结果，细胞凋亡促进了精子产生过程中其余细胞的成功发育。在精子发生过程中，支持细胞的支持能力逐渐增加。
