De Melo Dias Gisele Cristiane, Cassel Mônica, Oliveira De Jesus Lázaro Wender, Batlouni Sergio Ricardo, Borella Maria Inés
Fish Endocrinology Laboratory, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Reproduction Laboratory, Center of Aquaculture of São Paulo State University (CAUNESP), Jaboticabal, São Paulo, Brazil.
Anat Rec (Hoboken). 2017 Mar;300(3):589-599. doi: 10.1002/ar.23505. Epub 2016 Nov 7.
Prochilodus lineatus is an important representative of the order Characiformes and a species that offers great advantages to fish farming. Therefore, detailed knowledge of its reproductive biology can be applied to various fields of production and biotechnology. In this study, we have identified testicular germ cells during spermatogenesis and have evaluated the volumetric proportion of the testes occupied by structures of the tubular and intertubular compartments. In addition, the individual volume of type A spermatogonia was measured and used to estimate the mean number of these cells per testis. Gonads of adult P. lineatus males were extracted and fixed. Light and transmission electron microscopy were applied to fragments of three testicular regions. Histological, stereological, and morphometric analyses were performed. The stereological data suggest that components of the tubular and intertubular compartments of the P. lineatus testes present a uniform distribution in all three regions and therefore reflect regions with similar distributions of cell types. In addition, P. lineatus testes showed ∼0.6% of type A spermatogonia, as well as a predominance of cysts of primary spermatocytes and spermatids during the reproductive phase evaluated. The results from this study provide a better understanding of the morphology and structure of the testis and of the characterization of the type A spermatogonia in P. lineatus. The nuclear diameter of germ cells also decreases significantly during spermatogenesis. The data presented herein are the first of its kind for the order Characiformes and may be useful for future biotechnology studies on fish reproduction. Anat Rec, 300:589-599, 2017. © 2016 Wiley Periodicals, Inc.
细须脂鲤是脂鲤目重要的代表物种，也是一种在鱼类养殖方面具有很大优势的物种。因此，对其生殖生物学的详细了解可应用于生产和生物技术的各个领域。在本研究中，我们已识别出精子发生过程中的睾丸生殖细胞，并评估了睾丸中管状和管间腔结构所占的体积比例。此外，还测量了A型精原细胞的个体体积，并用于估计每个睾丸中这些细胞的平均数量。提取并固定成年雄性细须脂鲤的性腺。将光镜和透射电镜应用于三个睾丸区域的片段。进行了组织学、体视学和形态计量学分析。体视学数据表明，细须脂鲤睾丸的管状和管间腔成分在所有三个区域呈现均匀分布，因此反映了细胞类型分布相似的区域。此外，在评估的生殖阶段，细须脂鲤睾丸显示约0.6%的A型精原细胞，以及初级精母细胞和精子细胞囊肿占优势。本研究结果有助于更好地了解细须脂鲤睾丸的形态和结构以及A型精原细胞的特征。生殖细胞的核直径在精子发生过程中也显著减小。本文提供的数据在脂鲤目中尚属首次，可能对未来鱼类繁殖的生物技术研究有用。《解剖学记录》，300:589 - 599，2017年。© 2016威利期刊公司。
