Department of Hepatobiliary Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230039, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 5;188:633-639. doi: 10.1016/j.saa.2017.07.057. Epub 2017 Jul 31.
Small molecules possess large two-photon action cross sections (Φσ) are highly demanded for biological purpose. Herein, three novel terpyridine containing flexible amino diethylacetate organic small molecules (A1, A2 and A3) were rationally designed and their photophysical properties were investigated both experimentally and theoretically. The results revealed that the three chromophores possess large Φσ and remarkable Stokes' shift in high polar solvents, which are particularly benefit for further biological imaging application. One chromophore (A1) displayed an effective intracellular uptake against lung cancerous living cells A549. Colocalization studies suggested the internalized subcellular compartment was mitochondria. Consequently, chromophore A1 provides a promising platform to directly monitor mitochondria in living cells under two-photon confocal laser scanning microscopy.
小分子具有大的双光子作用截面（Φσ），因此在生物学方面有很高的需求。本文中，设计并合理合成了三种新型含灵活氨基二乙酸乙酯的三吡啶啉有机小分子（A1、A2 和 A3），并通过实验和理论研究对其光物理性质进行了研究。结果表明，这三种生色团具有大的Φσ和在高极性溶剂中显著的斯托克斯位移，这特别有利于进一步的生物成像应用。其中一个生色团（A1）对肺癌活细胞 A549 显示出有效的细胞内摄取。共定位研究表明，内化的亚细胞区室是线粒体。因此，生色团 A1 为在双光子共聚焦激光扫描显微镜下直接监测活细胞中线粒体提供了一个有前途的平台。
