Yeh Jih-Ching, Lo Dan-Yuan, Chang Shao-Kuang, Chou Chi-Chung, Kuo Hung-Chih
Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan.
Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Vet Rec. 2017 Sep 21;181(12):323. doi: 10.1136/vr.104023. Epub 2017 Aug 7.
(PM) can cause progressive atrophic rhinitis and suppurative bronchopneumonia in pigs. The present study performed antimicrobial susceptibility testing and serotype and genotype identification on the 62 PM strains isolated from the lungs of diseased pigs with respiratory symptoms. Antimicrobial susceptibility testing examined 13 antimicrobial agents (amoxicillin, cefazolin, doxycycline, flumequine, enrofloxacin, florfenicol, kanamycin, lincomycin, Linco-Spectin (lincomycin and spectinomycin), erythromycin, tylosin, tilmicosin and tiamulin). Antimicrobial resistance ratios were over 40% in all of the antimicrobial agents except for cefazolin. The highest levels of resistance (100%) were found for kanamycin, erythromycin and tylosin. The majority of isolated strains was serotype D:L6 (n=35) followed by A:L3 (n=17). Comparison of the antimicrobial resistance levels between the two serotypes showed that the antimicrobial resistance rates were higher in D:L6 than in A:L3 for all the tested antimicrobials except for tylosin and tilmicosin. For PM with (B), (T) or (42), the results showed no significant difference compared with non-resistance gene strains in phenotype. Pulsed-field gel electrophoresis genotyping using I restriction digestion of the genomic DNA demonstrated that there were 17 distinct clusters with a similarity of 85% or more, and the genotyping result was similar to that of serotyping. The results of the present study demonstrated that the PM isolated from diseased pigs in Taiwan was resistant to multiple antimicrobials, and the distribution of antimicrobial resistance was associated with pulsotype and serotype.
胸膜肺炎放线杆菌（PM）可导致猪的进行性萎缩性鼻炎和化脓性支气管肺炎。本研究对从有呼吸道症状的患病猪肺部分离出的62株PM菌株进行了药敏试验以及血清型和基因型鉴定。药敏试验检测了13种抗菌药物（阿莫西林、头孢唑林、强力霉素、氟甲喹、恩诺沙星、氟苯尼考、卡那霉素、林可霉素、林肯霉素-壮观霉素（林可霉素和壮观霉素）、红霉素、泰乐菌素、替米考星和替米考星）。除头孢唑林外，所有抗菌药物的耐药率均超过40%。卡那霉素、红霉素和泰乐菌素的耐药水平最高（100%）。分离出的大多数菌株为血清型D:L6（n = 35），其次是A:L3（n = 17）。两种血清型之间的耐药水平比较表明，除泰乐菌素和替米考星外，所有测试抗菌药物的D:L6血清型耐药率均高于A:L3血清型。对于携带（B）、（T）或（42）的PM，结果显示与无抗性基因菌株在表型上无显著差异。使用I对基因组DNA进行限制性消化的脉冲场凝胶电泳基因分型表明，有17个不同的簇，相似度为85%或更高，基因分型结果与血清分型结果相似。本研究结果表明，从台湾患病猪分离出的PM对多种抗菌药物耐药，抗菌药物耐药性的分布与脉冲型和血清型有关。
