Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Ridebanevej 3, DK-1870 Frederiksberg C, Denmark.
Vet Microbiol. 2011 Jun 2;150(3-4):354-61. doi: 10.1016/j.vetmic.2011.02.050. Epub 2011 Mar 3.
Pasteurella multocida is a widespread respiratory pathogen in pigs associated with atrophic rhinitis and contributing to aggravation of the pulmonary lesions. The aims of the present study were to characterize isolates of P. multocida from porcine bronchopneumonia by pulsed-field gel electrophoresis (PFGE), PCR based capsular typing and multilocus sequence typing (MLST) and to compare clonal complexes outlined with the type of histological lung lesions to investigate if a correlation between clonal lineages and lesions might exist. Isolates of P. multocida were obtained from cases of cranioventrally located porcine bronchopneumonia. All lung lesions were described and classified according to histological lesions. A total of 139 isolates, from lung (n=111), pericardial sac (n=21) and kidney (n=7) of 111 pigs were described using PFGE with ApaI as the restriction enzyme. Furthermore, 20 and 29 isolates were characterized by capsular serotyping and multilocus sequence typing, respectively. PFGE demonstrated 15 different clusters showing 50% or more similarity. All selected isolates were of capsular serotype A and only three main sequence types (ST) were detected among the isolates. Associations were not found between histopathology and clonal complexes of P. multocida. In conclusion, PFGE demonstrated a high diversity of genotypes of P. multocida associated with porcine bronchopneumonia. However, isolates obtained mainly belonged to few STs, indicating that isolates of P. multocida associated with porcine bronchopneumonia originates from a limited number of clonal lineages and therefore might have adapted to porcine hosts. No correlation was demonstrated between genotypes and types of lesions, and extra-pulmonary spreading was only rarely demonstrated.
多杀巴斯德菌是一种广泛存在于猪呼吸道的病原体，与萎缩性鼻炎有关，并导致肺部病变加重。本研究的目的是通过脉冲场凝胶电泳（PFGE）、基于 PCR 的荚膜分型和多位点序列分型（MLST）对猪支气管肺炎的多杀巴斯德菌分离株进行特征描述，并比较用组织学肺病变概述的克隆复合体，以研究克隆谱系与病变之间是否存在相关性。多杀巴斯德菌分离株从颅顶定位的猪支气管肺炎病例中获得。根据组织学病变对所有肺病变进行描述和分类。共描述了来自 111 头猪的肺（n=111）、心包囊（n=21）和肾脏（n=7）的 139 株分离株，使用 ApaI 作为限制酶的 PFGE。此外，通过荚膜血清分型和多位点序列分型分别对 20 株和 29 株分离株进行了特征描述。PFGE 显示 15 个不同的聚类，相似度达到 50%或更高。所有选定的分离株均为荚膜血清型 A，仅在分离株中检测到三种主要的序列型（ST）。未发现组织病理学与多杀巴斯德菌的克隆复合体之间存在关联。总之，PFGE 显示与猪支气管肺炎相关的多杀巴斯德菌基因型具有高度多样性。然而，获得的分离株主要属于少数 ST，表明与猪支气管肺炎相关的多杀巴斯德菌分离株起源于有限数量的克隆谱系，因此可能已经适应了猪宿主。未证明基因型与病变类型之间存在相关性，很少证明肺外传播。
