Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Sci Rep. 2017 Aug 7;7(1):7407. doi: 10.1038/s41598-017-06513-4.
Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP1 expression in the recombinant infectious clone, on which the C-terminus of NS1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. The result suggested that NP1 facilitated the replication of viral genome but was not necessary, which is different from the minute virus of canines, where NP1 is essential for viral replication. Further studies showed that clinical mutations in the NP1 region did not affect viral genome replication, and UP1 promoted viral DNA replication. Our results suggested that the C-terminus of NS1 is important for viral replication and may be a target for regulating the replication of the viral genome.
人博卡病毒 1（HBoV1）是细小病毒科博卡病毒属的自主细小病毒。多功能核蛋白 NP1 参与病毒复制。在本研究中，我们发现 NS1 末端的突变影响了 NP1 在病毒复制中的功能。敲除基于鼻咽抽吸物临床样本构建的重组传染性克隆中 NP1 的表达，导致病毒基因组的复制程度不同程度地降低。结果表明，NP1 有助于病毒基因组的复制，但不是必需的，这与犬细小病毒不同，犬细小病毒的 NP1 是病毒复制所必需的。进一步的研究表明，NP1 区的临床突变不影响病毒基因组的复制，而 UP1 促进了病毒 DNA 的复制。我们的结果表明，NS1 的 C 末端对病毒复制很重要，可能是调节病毒基因组复制的靶点。
