Tang Jielin, Chen Sijie, Deng Yijun, Liu Junjun, Huang Dan, Fu Muqing, Xue Bao, Liu Canyu, Wu Chunchen, Wang Fan, Zhou Yuan, Yang Qi, Chen Xinwen
Guangzhou National Laboratory, Guangzhou, China.
Guangzhou Medical University, Guangzhou, China.
J Virol. 2025 Feb 25;99(2):e0153924. doi: 10.1128/jvi.01539-24. Epub 2025 Jan 23.
Human bocavirus 1 (HBoV1) has appeared as an emerging pathogen, causing mild to life-threatening respiratory tract infections, acute otitis media, and encephalitis in young children and immunocompromised individuals. The lack of cell lines suitable for culturing replicative viruses hinders research on HBoV1. Here, we characterized the susceptibility to HBoV1 of 29 human and 7 animal cell lines, and identified a permissive cell line, MA104. The complete HBoV1 life cycle was achieved in MA104 cells, including viral entry, complete replication, and infectious progeny virion production. Additionally, the suppression of the interferon pathway facilitated the viral genome replication in MA104 cells. RNA-sequencing showed that innate immunity, inflammation, the PI3K-Akt and MAPK signaling pathways, and the cellular membrane system were mobilized in response to HBoV1 infection. Overall, our study is the first to identify a cell line, MA104, that supports the complete HBoV1 life cycle, which will promote research on HBoV1 virology and pathogenesis and benefit drug and vaccine development.IMPORTANCEHBoV1 is an emerging pathogen that mainly causes respiratory tract infections, while the lack of cell lines suitable for culture replicative viruses hindered research on HBoV1. Here, we identify a permissive cell line for HBoV1 infection, MA104, and reveal that the complete life cycle of HBoV1 was supported in MA104 cells. Our findings provide a suitable cell model for the study of HBoV1 and explore its application for antiviral drug evaluation, which is vital for research on HBoV1 virology and pathogenesis, as well as for drug and vaccine development.
人博卡病毒1型（HBoV1）已成为一种新出现的病原体，可导致幼儿和免疫功能低下个体出现从轻度到危及生命的呼吸道感染、急性中耳炎和脑炎。缺乏适合培养复制性病毒的细胞系阻碍了对HBoV1的研究。在此，我们对29种人类细胞系和7种动物细胞系对HBoV1的敏感性进行了表征，并鉴定出一种允许性细胞系MA104。HBoV1的完整生命周期在MA104细胞中得以实现，包括病毒进入、完全复制以及感染性子代病毒粒子的产生。此外，干扰素途径的抑制促进了MA104细胞中病毒基因组的复制。RNA测序表明，先天免疫、炎症、PI3K - Akt和MAPK信号通路以及细胞膜系统在对HBoV1感染的反应中被激活。总体而言，我们的研究首次鉴定出一种支持HBoV1完整生命周期的细胞系MA104，这将促进对HBoV1病毒学和发病机制的研究，并有利于药物和疫苗的开发。
重要性
HBoV1是一种主要引起呼吸道感染的新出现病原体，而缺乏适合培养复制性病毒的细胞系阻碍了对HBoV1的研究。在此，我们鉴定出一种对HBoV1感染具有允许性的细胞系MA104，并揭示MA104细胞支持HBoV1的完整生命周期。我们的发现为HBoV1的研究提供了一个合适的细胞模型，并探索了其在抗病毒药物评估中的应用，这对于HBoV1病毒学和发病机制的研究以及药物和疫苗的开发至关重要。
